Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies.
Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies.
复制标题
稳定哺乳动物细胞系中视紫红质及其突变体的表达和纯化：在 NMR 研究中的应用。
DOI：
10.1042/bst0270950
复制
发表时间：
1999
影响因子：
3.9
通讯作者：
Khorana,HG
中科院分区：
文献类型：
--
作者：
Reeves,PJ;Klein-Seetharaman,J;Getmanova,EV;Eilers,M;Loewen,MC;Smith,SO;Khorana,HG
从膜蛋白中获取高分辨率的结构信息仍然是一个巨大的挑战。一个主要的限制，特别是对真核蛋白，包括G-蛋白偶联受体家族，往往是少量的纯化蛋白可用于分析。虽然事实并非如此
The acquisition of high-resolution structural information from integral membrane proteins continues to present a formidable challenge. A major limitation, particularly for eukaryotic proteins including the G-protein-coupled receptor family, is often the small amounts of purified protein available for analvsis. Although this is not the case