Remote control of photosynthetic genes by the mitochondrial respiratory chain.
Remote control of photosynthetic genes by the mitochondrial respiratory chain.
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DOI：
10.1111/j.1365-313x.2006.02839.x
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发表时间：
2006-09
期刊：
影响因子：
--
通讯作者：
M. Matsuo;J. Obokata
中科院分区：
文献类型：
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作者：
M. Matsuo;J. Obokata
在本文中，我们研究了线粒体呼吸是否对单细胞藻类莱茵衣藻光合器官的生物发生有影响。当在黑暗中醋酸盐激活呼吸作用时，核编码的光合基因的 mRNA 就会被诱导。这种诱导并未发生在用呼吸抑制剂处理的细胞或呼吸突变体中。氧化磷酸化解偶联剂不会抑制这种 mRNA 诱导；相反，它是随着呼吸电子传递（RET）的增加而增强的。植物和藻类线粒体有两种 RET 途径：细胞色素途径和旁路途径。前一种途径的抑制剂抑制 mRNA 的诱导，而后一种途径的抑制剂则增强它。总而言之，这些表明光合基因 mRNA 是响应细胞色素途径的激活而被诱导的。这种 RET 响应性诱导类似于光合基因 mRNA 的光合电子传递 (PET) 响应性诱导（Matsuo 和 Obokata，Plant Cell Physiol. 43, 1189）。 PET响应性诱导发生在光自养和混合营养条件下，而RET响应性诱导发生在混合营养和暗异养条件下。这些结果表明，光合作用基因的调节系统响应于光合作用和呼吸之间主要能量来源的变化而在叶绿体PET依赖型和线粒体RET依赖型之间变化。
In this paper, we study whether mitochondrial respiration has an impact on the biogenesis of photosynthetic apparatus in the unicellular alga, Chlamydomonas reinhardtii. When respiration was activated by acetate in the dark, mRNAs of nuclear-encoded photosynthetic genes were induced. This induction did not occur in the cells treated with respiration inhibitors or in respiration mutants. An uncoupler of oxidative phosphorylation did not inhibit this mRNA induction; rather, it enhanced it in response to the increase in respiratory electron transport (RET). Plant and algal mitochondria have two RET pathways: the cytochrome pathway and the alternative pathway. Inhibitors of the former pathway inhibited mRNA induction, but inhibitors of the latter enhanced it. Taken together, these indicate that photosynthetic gene mRNAs are induced in response to activation of the cytochrome pathway. This RET-responsive induction is analogous to the photosynthetic electron transport (PET)-responsive induction of photosynthetic gene mRNAs (Matsuo and Obokata, Plant Cell Physiol. 43, 1189). PET-responsive induction occurred in photo-autotrophic and mixotrophic conditions, while RET-responsive induction occurred in mixotrophic and dark heterotrophic conditions. These results indicate that the regulatory system of photosynthetic genes changes between chloroplastic PET-dependent type and mitochondrial RET-dependent type in response to shifts in the dominant energy source between photosynthesis and respiration.