Biofilm-forming ability and resistance to industrial disinfectants of Staphylococcus aureus isolated from fishery products
Biofilm-forming ability and resistance to industrial disinfectants of Staphylococcus aureus isolated from fishery products
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DOI：
10.1016/j.foodcont.2013.09.029
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发表时间：
2014-05
期刊：
影响因子：
6
通讯作者：
D. Vázquez-Sánchez;M. L. Cabo;P. S. Ibusquiza;J. Rodríguez-Herrera
中科院分区：
文献类型：
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作者：
D. Vázquez-Sánchez;M. L. Cabo;P. S. Ibusquiza;J. Rodríguez-Herrera
研究了26株分离自水产品的金黄色葡萄球菌在不锈钢表面的粘附和生物膜形成能力。所有菌株在25 °C下5小时后达到高于104 CFU/cm 2的计数。大多数菌株的生物膜形成能力也高于金黄色葡萄球菌ATCC 6538 -杀菌标准试验中的参考菌株-通过结晶紫染色。此外，食品加工可能产生选择性压力，具有高生物膜形成能力的菌株更可能在高度处理和加工的产品中发现。次氯酸钠（NaClO）和过氧乙酸（PAA）对生物膜和渗透性对应物的最小生物膜根除浓度（MBEC）也进行了检查和最低杀菌浓度（MBC）。在所有情况下，生物膜显示出比嗜酸性细胞更高的抗微生物剂抗性。然而，MBEC和MBC之间没有发现相关性，这可能是由于菌株之间生物膜细胞外基质（组成、含量和结构）的差异。BAC抗性随生物膜老化而增加。一般来说，生物膜的形成似乎减弱了低温对BAC抗性的影响。PAA被发现是最有效的对生物膜和反渗透细胞，其次是NaClO和BAC。每种杀生物剂的耐药性并不遵循相同的顺序，这表明需要在杀菌活性的标准测试中使用广泛的菌株，以确保正确应用消毒剂。制造商推荐的BAC、PAA和NaClO消毒食品接触表面的剂量低于在食品工业中常见的一些环境条件下完全去除生物膜（即MBEC）的剂量，这对杀菌标准测试提出了质疑，并促进了对生物膜去除新策略的研究。
Adhesion and biofilm-forming ability of twenty sixS. aureusstrains previously isolated from fishery products on stainless steel was assessed. All strains reached counts higher than 104CFU/cm2after 5 h at 25 °C. Most strains also showed a biofilm-forming ability higher thanS. aureusATCC 6538 – reference strain in bactericidal standard tests – by crystal violet staining. In addition, it seems that food-processing could have produced a selective pressure and strains with a high biofilm-forming ability were more likely found in highly handled and processed products.The efficacy of the industrial disinfectants benzalkonium chloride (BAC), sodium hypochlorite (NaClO) and peracetic acid (PAA) against biofilms and planktonic counterparts was also examined in terms of minimum biofilm eradication concentration (MBEC) and minimum bactericidal concentration (MBC), respectively. Biofilms showed an antimicrobial resistance higher than planktonic cells in all cases. However, no correlation was found between MBEC and MBC, likely due to differences in biofilm extracellular matrix (composition, content and architecture) between strains. BAC resistance increased as biofilms aged. Generally, biofilm formation seemed to attenuate the effect of low temperatures on BAC resistance. PAA was found to be most effective against both biofilms and planktonic cells, followed by NaClO and BAC. Resistance did not follow the same order for each biocide, which remarks the need of using a wide collection of strains in standard tests of bactericidal activity to ensure a proper application of disinfectants. Doses recommended by manufacturers for BAC, PAA and NaClO to disinfect food-contact surfaces were lower than doses for complete biofilm removal (i.e. MBEC) under some environmental conditions common in the food industry, which questions bactericidal standard tests and promotes the search for new strategies for biofilm removal.