Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress
Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress
复制标题
DOI：
10.1016/j.placenta.2010.08.003
复制
发表时间：
2010-11-01
期刊：
影响因子：
3.8
通讯作者：
Nakashima, E.
中科院分区：
文献类型：
--
作者：
Nishimura, T.;Sai, Y.;Nakashima, E.
本研究旨在阐明暴露于高渗条件下的条件永生化合体滋养层细胞系（TR-TBT 18 d-1）诱导的细胞保护机制。高渗诱导TR-TBT 18 d-1细胞凋亡，但这被阻断添加1 mM牛磺酸的培养基。TauT-敲低使用siRNA显示，TauT是一个主要的贡献者，牛磺酸的摄取TR-TBT 18 d-1细胞，至少在正常条件下。高渗条件下培养的TR-TBT 18 d-1细胞对[（3）H]牛磺酸和[（14）C]甜菜碱的摄取较正常条件下增加。TauT、BGT-1、ATA 2和HSP 70 mRNA在高渗条件下表达上调，而OCTN 2、ENT 1和CNT 1 mRNA表达下调。[（3）H]牛磺酸摄取被TauT抑制剂如亚牛磺酸和β-丙氨酸强烈抑制。MeAIB是系统A特异性底物，可抑制高渗胁迫诱导的[（14）C]甜菜碱摄取。这些结果表明，TauT和系统A发挥细胞保护作用的合胞体滋养细胞暴露于高渗应激。(C)2010爱思唯尔有限公司版权所有。
The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [(3)H]taurine and [(14)C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [(3)H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and beta-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [(14)C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress. (C) 2010 Elsevier Ltd. All rights reserved.