Aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro antitumor activity
Aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro antitumor activity
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DOI：
10.1016/j.nutres.2012.11.005
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发表时间：
2013-01-01
影响因子：
4.5
通讯作者：
Roesch-Ely, M.
中科院分区：
文献类型：
--
作者：
Finimundy, T. C.;Gambato, G.;Roesch-Ely, M.
蘑菇提取物越来越多地作为膳食补充剂出售，因为它们具有多种特性，包括增强免疫功能和抗肿瘤活性。我们假设蘑菇提取物中存在的可溶性极性物质可能具有抗氧化和抗癌特性。这份报告表明，巴西的香菇和Pleopulsajor-caju水提取物对人肿瘤细胞系喉癌（Hep-2）和宫颈腺癌（HeLa）的增殖发挥抑制活性。在使用3种不同的蘑菇提取温度（4 ° C、22 ° C和50 ° C）后测定细胞活力。平行进行的生化分析表明，在所有的提取温度调查香菇提取物中的多酚含量较高。香菇提取物对2，2-二苯基-1-苦肼基自由基的清除能力较强。超氧化物歧化酶样活性在两种试验提取物的组间没有显示出统计学显著差异，并且过氧化氢酶样活性在4 ℃时随着香菇提取物而增加。在22 ℃下来自青豆提取物的细胞毒活性的结果显示，对于Hep-2和HeLa，半最大抑制浓度值分别为0.64% +/-0.02%和0.25% +/-0.02%。香菇提取物在22 ℃下具有更高的细胞毒活性，Hep-2的半数最大抑制浓度值为0.78% +/- 0.02%，HeLa为0.57% +/- 0.01%。用任一种提取物处理后，通过Giemsa染色证实细胞中的实质性形态改变，表明随着提取物浓度的增加，增殖抑制和凋亡诱导。这些结果表明，巴西香菇和P sajor-caju蘑菇的水提取物是抗氧化剂和抗癌化合物的潜在来源。然而，需要进一步的调查，以利用其有价值的治疗用途，并阐明其作用模式。(C)2013 Elsevier Inc. All rights reserved.
Mushroom extracts are increasingly sold as dietary supplements because of several of their properties, including the enhancement of immune function and antitumor activity. We hypothesized that soluble polar substances present in mushroom extracts may show antioxidant and anticancer properties. This report shows that Brazilian aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exert inhibitory activity against the proliferation of the human tumor cell lines laryngeal carcinoma (Hep-2) and cervical adenocarcinoma (HeLa). Cell viability was determined after using 3 different temperatures (4 degrees C, 22 degrees C, and 50 degrees C) for mushroom extraction. Biochemical assays carried out in parallel indicated higher amounts of polyphenols in the L edodes extracts at all extraction temperatures investigated. The scavenging ability of the 2,2-diphenyl-1-picrylhydrazyl radical showed higher activity for L edodes extracts. Superoxide dismutase like activity showed no statistically significant difference among the groups for the 2 tested extracts, and catalase-like activity was increased with the L edodes extracts at 4 degrees C. The results for the cytotoxic activity from P sajor-caju extracts at 22 degrees C revealed the half maximal inhibitory concentration values of 0.64% +/- 0.02% for Hep-2 and 0.25% +/- 0.02% for HeLa. A higher cytotoxic activity was found for the L edodes extract at 22 degrees C, with half maximal inhibitory concentration values of 0.78% +/- 0.02% for Hep-2 and 0.57% +/- 0.01% for HeLa. Substantial morphological modifications in cells were confirmed by Giemsa staining after treatment with either extract, suggesting inhibition of proliferation and induction of apoptosis with increasing extract concentrations. These results indicate that the aqueous extracts of Brazilian L edodes and P sajor-caju mushrooms are potential sources of antioxidant and anticancer compounds. However, further investigations are needed to exploit their valuable therapeutic uses and to elucidate their modes of action. (C) 2013 Elsevier Inc. All rights reserved.