Dissemination of wine-related yeasts by migratory birds
Dissemination of wine-related yeasts by migratory birds
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DOI：
10.1111/j.1758-2229.2011.00310.x
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发表时间：
2012-02-01
影响因子：
3.3
通讯作者：
Moschetti, Giancarlo
中科院分区：
文献类型：
--
作者：
Francesca, Nicola;Canale, Domenica E.;Moschetti, Giancarlo
目前的工作是为了评估候鸟在酵母环境传播中的贡献。四个网站（马扎拉德尔瓦洛，兰佩杜萨，乌斯蒂卡和利诺萨），代表在西西里的主要中途停留点，进行了分析，在春季和秋季的鸟类迁徙和349只鸟（属于10个家庭）进行了环形和酵母菌的存在进行了分析。采用5.8S rRNA基因和26 S rRNA基因的限制性片段长度多态性（RFLP）和26 S rRNA基因的D1/D2区序列测定等多基因型分析方法，对125株酵母菌进行了分离鉴定，共鉴定出18种酵母菌，包括采用三种技术在菌株水平上表征的技术相关酿酒酵母（interdelta分析、基于三个基因单独扩增的微卫星分析和多态性微卫星位点的微卫星多重PCR）。对活S.从摄入接种的饲料开始，在鸟类中观察约12小时的酿酒酵母，可以得出结论，具有技术潜力的酵母在迁移过程中传播。
The present work was undertaken to evaluate the contribution of migratory birds in the environmental dissemination of yeasts. Four sites (Mazara del Vallo, Lampedusa, Ustica and Linosa), representing the main stop-over points in Sicily, were analysed during spring and autumnal bird migration and 349 birds (belonging to 10 families) were ringed and analysed for yeast presence. A total of 125 yeasts were isolated and identified by a multiple genotypic approach, consisting of restriction fragment length polymorphism (RFLP) of 5.8S rRNA gene and 26S rRNA and sequencing of D1/D2 domain of the 26S rRNA gene, which resulted in the recognition of 18 species, including the technological relevant Saccharomyces cerevisiae which were characterized at strain level applying three techniques (interdelta analysis, mini-satellite analysis based on the separate amplification of three genes and microsatellite multiplex PCR of polymorphic microsatellite loci). The evaluation of the persistence of living S. cerevisiae in birds for about 12 h from ingestion of inoculated feed allowed the conclusion that yeasts with technological potential are disseminated during migration.