Phosphate regulation of gene expression in Vibrio parahaemolyticus
Phosphate regulation of gene expression in Vibrio parahaemolyticus
复制标题
副溶血弧菌基因表达的磷酸盐调控
DOI：
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发表时间：
1987
影响因子：
3.2
通讯作者：
M. Silverman
中科院分区：
文献类型：
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作者：
L. McCarter;M. Silverman
副溶血弧菌中主要外膜蛋白 OmpP 的合成是通过在缺乏磷酸盐的培养基中生长来诱导的。编码该蛋白质的基因 ompP 被克隆。大肠杆菌中 OmpP 的合成受到磷酸盐可用性的调节，而这种控制需要大肠杆菌 pho 调节基因的功能。对通过转座子 mini-Mulux 诱变构建的基因融合菌株的分析表明，ompP 在副溶血弧菌中受到转录调控。在含有大的线性聚磷酸盐作为磷酸盐源的培养基中观察到具有ompP缺陷的菌株的生长受损。这一证据和其他证据表明，OmpP 充当磷酸盐进入细胞的孔蛋白通道。许多其他蛋白质或活性是由磷酸盐限制诱导的，包括溶血素、磷脂酶 C 和磷酸酶活性。鉴定并克隆了控制磷酸盐调节基因表达的调节位点。该从副溶血弧菌克隆的调节位点被证明可以补充具有 pho 调节基因缺陷的大肠杆菌菌株。
The synthesis of a major outer membrane protein, OmpP, in Vibrio parahaemolyticus was induced by growth in media deficient in phosphate. The gene, ompP, encoding this protein was cloned. Synthesis of OmpP in Escherichia coli was regulated by the availability of phosphate, and this control required the function of pho regulatory genes of E. coli. Analysis of gene fusion strains constructed by mutagenesis with transposon mini-Mulux revealed that ompP was transcriptionally regulated in V. parahaemolyticus. Impaired growth of a strain with an ompP defect was observed in media which contained large linear polyphosphates as the phosphate source. This and other evidence suggested that OmpP functions as a porin channel for the entry of phosphate into the cell. A number of other proteins or activities were induced by phosphate limitation including hemolysin, phospholipase C, and phosphatase activities. A regulatory locus controlling expression of phosphate-regulated genes was identified and cloned. This regulatory locus cloned from V. parahaemolyticus was shown to complement E. coli strains with defects in pho regulatory genes.