Detection of aberrations of ubiquitin-conjugating enzyme E2C gene (UBE2C) in advanced colon cancer with liver metastases by DNA microarray and two-color FISH
Detection of aberrations of ubiquitin-conjugating enzyme E2C gene (UBE2C) in advanced colon cancer with liver metastases by DNA microarray and two-color FISH
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DOI：
10.1016/j.cancergencyto.2005.12.011
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发表时间：
2006-07-01
影响因子：
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通讯作者：
Asai, Satoshi
中科院分区：
文献类型：
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作者：
Takahashi, Yasuo;Ishii, Yukimoto;Asai, Satoshi
使用DNA微阵列，研究了9例晚期结直肠癌患者的原发肿瘤、肝转移灶和配对正常组织中1,700个基因的表达谱。在原发性肿瘤中，20个基因上调，只有一个基因下调。在肝转移瘤中，39个基因表达上调，只有3个基因表达下调。原发灶与肝转移灶之间的基因表达无明显差异。在原发性肿瘤和肝转移瘤中最高度过表达的基因是泛素结合酶E2 C基因（UBE 2C），位于20q13.1。此外，双色荧光原位杂交分析使用区域20q13.1和染色体20着丝粒的探针显示，在20q13.1扩增发生在5/10（50%）结肠癌。基因表达水平和FISH结果之间的比较显示，UBE 2C表达显着改变20 q13.1扩增，表明基因组扩增作为一种机制，增加UBE 2C表达。我们的研究结果显示UBE 2C基因表达水平和基因座拷贝数的畸变表明，该基因可能在导致晚期结肠癌肝转移的肿瘤进展中起重要作用。(c)2006年爱思唯尔公司All rights reserved.
Using DNA microarrays, the expression profiles of 1,700 genes in the primary tumor, liver metastases and paired normal tissue obtained from nine patients with advanced colorectal cancer was studied. Twenty genes were upregulated and only one gene was downregulated in the primary tumors. In the liver metastases, 39 genes were upregulated and only three genes were downregulated. There was no significant difference in gene expression between the primary tumors and the liver metastases. The most highly overexpressed gene in both the primary tumors and the liver metastases was the ubiquitin-conjugating enzyme E2C gene (UBE2C), located at 20q13.1. Additionally, two-color FISH analysis using probes for the region 20q13.1 and the chromosome 20 centromere revealed that amplification at 20q13.1 had occurred in 5 of 10 (50%) colon cancers. Comparison between the levels of gene expression and FISH results revealed that UBE2C expression is significantly changed by amplification at 20q13.1, suggesting genomic amplification as one mechanism of increased UBE2C expression. Our results showing aberrations in levels of gene expression and locus copy number of UBE2C suggest that this gene may play an important role in tumor progression leading to advanced colon cancer with liver metastasis. (c) 2006 Elsevier Inc. All rights reserved.