Liquid-like Solids Support Cells in 3D
Liquid-like Solids Support Cells in 3D
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DOI：
10.1021/acsbiomaterials.6b00218
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发表时间：
2016-10-01
影响因子：
5.8
通讯作者：
Angelini, Thomas E.
中科院分区：
文献类型：
--
作者：
Bhattacharjee, Tapomoy;Gil, Carmen J.;Angelini, Thomas E.
组织工程的需求已经推动了3D组织培养技术的大量研究工作，最近，在3D打印。在所有细胞生物学中更广泛地使用3D组织培养技术的需求是众所周知的，但是向3D的过渡受到2D培养材料、测定和方案的方便性、有效性和普遍性以及缺乏这些工具的3D对应物的阻碍。有趣的是，3D生物打印研究的进展和发现可能为3D文化的发展提供所需的技术支持。在这里，我们研究了一种用于3D打印多细胞结构的集成方法，同时使用相同的平台进行3D细胞培养，实验和检测开发。我们采用了一种由填充的颗粒状微凝胶制成的类液体固体（LLS）材料，该材料在集中施加应力的情况下局部和暂时地流化，并在施加的应力被移除后自发地固化。这些流变学特性使多细胞结构的3D打印以及细胞结构或分散细胞的生长和扩张成为可能。LLS的运输特性允许分子扩散，用于递送营养物或小分子，用于基于荧光的测定。在这里，我们测量了LLS培养基中11种不同细胞类型的活力，我们使用其中几种细胞类型3D打印了许多结构，并探索了分子时间释放试验中的运输特性。
The demands of tissue engineering have driven a tremendous amount of research effort in 3D tissue culture technology and, more recently, in 3D printing. The need to use 3D tissue culture techniques more broadly in all of cell biology is well-recognized, but the transition to 3D has been impeded by the convenience, effectiveness, and ubiquity of 2D culture materials, assays, and protocols, as well as the lack of 3D counterparts of these tools. Interestingly, progress and discoveries in 3D bioprinting research may provide the technical support needed to grow the practice of 3D culture. Here we investigate an integrated approach for 3D printing multicellular structures while using the same platform for 3D cell culture, experimentation, and assay development. We employ a liquid-like solid (LLS) material made from packed granular-scale microgels, which locally and temporarily fluidizes under the focused application of stress and spontaneously solidifies after the applied stress is removed. These rheological properties enable 3D printing of multicellular structures as well as the growth and expansion of cellular structures or dispersed cells. The transport properties of LLS allow molecular diffusion for the delivery of nutrients or small molecules for fluorescence-based assays. Here, we measure viability of 11 different cell types in the LLS medium, we 3D print numerous structures using several of these cell types, and we explore the transport properties in molecular time-release assays.