Radial glial cell line C6-R integrates preferentially in adult white matter and facilitates migration of coimplanted neurons in vivo.
Radial glial cell line C6-R integrates preferentially in adult white matter and facilitates migration of coimplanted neurons in vivo.
复制标题
放射状胶质细胞系 C6-R 优先整合到成人白质中，并促进体内共同植入的神经元的迁移。
DOI：
10.1006/exnr.2000.7620
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Grumet,M
中科院分区：
文献类型：
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作者：
Hormigo,A;McCarthy,M;Nothias,JM;Hasegawa,K;Huang,W;Friedlander,DR;Fischer,I;Fishell,G;Grumet,M
C6-R是来源于C6神经胶质瘤细胞的细胞系，其表现出放射状神经胶质细胞的关键特性，包括在培养物中支持神经元迁移的能力。为了探索其在体内促进神经元迁移的潜在用途，我们分析了C6-R细胞在完整和损伤的成年大鼠CNS中的行为。在胼胝体压部植入后6-11天，观察到绿色荧光蛋白标记的C6-R细胞主要在大脑的胼胝体或海马中，并且在脊髓中，它们在白色物质中比在灰质中迁移更广泛。为了确定C6-R细胞是否保留其在体内促进神经元迁移的能力，将它们与标记的神经元共注射到成人脑中。当大鼠胚胎神经元与C6-R细胞共植入时，神经元和C6-R细胞共迁移的体积比单独的神经元或神经元与成纤维细胞共植入的体积大得多。在鹅膏蕈氨酸预损伤的脑中，C6-R细胞以及共同植入的神经元广泛分布在病变部位并迁移到邻近的脑组织中，而仅移植神经元主要限于病变部位。结果表明，放射状胶质细胞系可以作为神经元迁移的支架，可能有助于神经移植和再生的实验模型的发展。
C6-R is a cell line derived from C6 glioma cells that exhibits key properties of radial glia including the ability to support neuronal migration in culture. To explore its potential use in promoting neuronal migration in vivo, we analyzed the behavior of C6-R cells in the intact and injured adult rat CNS. At 6–11 days postimplantation at the splenium of the corpus callosum, green fluorescent protein-labeled C6-R cells were observed primarily in either the corpus callosum or the hippocampus in the brain, and in the spinal cord they migrated more extensively in the white matter than in the grey matter. To determine whether C6-R cells retain their ability to promote neuronal migration in vivo, they were coinjected with labeled neurons into adult brain. When rat embryonic neurons were coimplanted with C6-R cells, the neurons and C6-R cells comigrated through a much larger volume than neurons alone or neurons coimplanted with fibroblasts. In brains preinjured with ibotenic acid, C6-R cells as well as coimplanted neurons distributed widely within the lesion site and migrated into adjacent brain tissue, while transplants with neurons alone were restricted primarily to the lesion site. The results suggest that radial glial cell lines can serve as a scaffold for neuronal migration that may facilitate development of experimental models for neural transplantation and regeneration.