Optical manipulation: advances for biophotonics in the 21st century.
Optical manipulation: advances for biophotonics in the 21st century.
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DOI：
10.1117/1.jbo.26.7.070602
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发表时间：
2021-07
影响因子：
3.5
通讯作者：
Dholakia K
中科院分区：
文献类型：
--
作者：
Corsetti S;Dholakia K
重要性：光学捕获是一种能够施加微小力的技术，已应用于从单分子到微生物的研究。目的：这一观点的目标是突出在过去十年中在这一领域的一些主要进展是相关的生物医学观众。方法：首先，直接确定力的光镊和光学和声学陷阱的组合，它允许跨不同的长度尺度的研究，进行了讨论。然后，综述了利用光学力直接捕获单分子，甚至单病毒和单细胞的研究进展。最后，这种方法在生物光子学的未来方向进行了讨论。结果如下：在世纪，光学操作扩展了其独特的功能，不仅可以对单分子和单细胞进行更详细的研究，还可以对更复杂的生命系统进行研究，使我们对重要的生物活动有了进一步的了解。结论：光学力在生物医学领域发挥了重要作用，导致了特殊的新生物学突破。光学捕获领域的不断进步必将导致进一步的开发，包括令人兴奋的体内实验。
Significance: Optical trapping is a technique capable of applying minute forces that has been applied to studies spanning single molecules up to microorganisms. Aim: The goal of this perspective is to highlight some of the main advances in the last decade in this field that are pertinent for a biomedical audience. Approach: First, the direct determination of forces in optical tweezers and the combination of optical and acoustic traps, which allows studies across different length scales, are discussed. Then, a review of the progress made in the direct trapping of both single-molecules, and even single-viruses, and single cells with optical forces is outlined. Lastly, future directions for this methodology in biophotonics are discussed. Results: In the 21st century, optical manipulation has expanded its unique capabilities, enabling not only a more detailed study of single molecules and single cells but also of more complex living systems, giving us further insights into important biological activities. Conclusions: Optical forces have played a large role in the biomedical landscape leading to exceptional new biological breakthroughs. The continuous advances in the world of optical trapping will certainly lead to further exploitation, including exciting in-vivo experiments.