Size-exclusion chromatography in structural analysis of intrinsically disordered proteins.
Size-exclusion chromatography in structural analysis of intrinsically disordered proteins.
复制标题
本质无序蛋白质结构分析中的尺寸排阻色谱法。
作者：
V. Uversky
凝胶过滤层析，又称尺寸排除层析(SEC)或凝胶渗透层析，是分析内在无序蛋白质(IDPs)结构和构象的有用工具。SEC可用于估计给定IDP的流体动力学尺寸、评估缔合状态、分析IDP与结合伙伴的相互作用以及诱导折叠研究。它还可以用来根据流体力学尺寸对IDP构象进行物理分离，从而为独立分析其物理化学性质提供了独特的可能性。
Gel-filtration chromatography, also known as size-exclusion chromatography (SEC) or gel-permeation chromatography, is a useful tool for structural and conformational analyses of intrinsically disordered proteins (IDPs). SEC can be utilized for the estimation of the hydrodynamic dimensions of a given IDP, for evaluation of the association state, for the analysis of IDP interactions with binding partners, and for the induced folding studies. It also can be used to physically separate IDP conformers based on their hydrodynamic dimensions, thus providing a unique possibility for the independent analysis of their physicochemical properties.
DOI：
10.1016/s0162-3109(00)00244-7
发表时间：
2000-09-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
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作者：
Li, BQ;Fu, T;Wang, JM
通讯作者：
Wang, JM