PCR-mediated Identification of the Newly Emerging Pathogen Lactococcus garvieae Serotype II from Seriola quinqueradiata and S. dumerili
PCR-mediated Identification of the Newly Emerging Pathogen Lactococcus garvieae Serotype II from Seriola quinqueradiata and S. dumerili
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DOI：
10.3147/jsfp.52.46
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发表时间：
2017-03-01
期刊：
影响因子：
0.6
通讯作者：
Nishiki, Issei
中科院分区：
文献类型：
--
作者：
Ohbayashi, Kazuya;Oinaka, Daisaku;Nishiki, Issei
新出现的病原菌Lactococcus garvieae血清型II与L. garvieae血清型I，已从养殖的Seriola quinqueradiata和S.杜梅里利。为了区分这两种血清型，设计了靶向glxR-argS基因间区域的引物组。使用该引物组进行PCR扩增出不同大小的片段：血清II型为1，285 bp，血清I型为285 bp。因此，该引物组能够PCR介导鉴定从日本海洋鱼类中分离的血清型II菌株。
The newly emerging pathogen Lactococcus garvieae serotype II, which appears to be morphologically and biochemically indistinguishable from L. garvieae serotype I, has been isolated from farmed Seriola quinqueradiata and S. dumerili. To distinguish the two serotypes, a primer set was designed to target the glxR-argS intergenic region. PCR with the primer set amplified different-sized fragments: 1,285 bp for serotype II and 285 bp for serotype I. Therefore, the primer set enables the PCR-mediated identification of serotype II strains isolated from marine fish species in Japan.