THE AMINO-ACID-SEQUENCE OF THE VARIABLE REGION OF A CARBOHYDRATE-CONTAINING AMYLOID FIBRIL PROTEIN EPS (IMMUNOGLOBULIN LIGHT CHAIN, TYPE-LAMBDA)
THE AMINO-ACID-SEQUENCE OF THE VARIABLE REGION OF A CARBOHYDRATE-CONTAINING AMYLOID FIBRIL PROTEIN EPS (IMMUNOGLOBULIN LIGHT CHAIN, TYPE-LAMBDA)
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DOI：
10.1515/bchm3.1985.366.2.617
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
HUSBY, G
中科院分区：
文献类型：
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作者：
TOFT, KG;SLETTEN, K;HUSBY, G
IG的含碳水化合物的淀粉样原纤维蛋白EPS的可变区的氨基酸序列。阐明了轻链起源。从患有IgM型免疫细胞恶液质的患者（EPS）的肝脏中分离出该蛋白质。发现该蛋白质的分子量约为20 kDa [Kdalton]，包括与其连接的寡糖链。氨基酸序列测定涉及用BNPS-粪臭素、胰蛋白酶和嗜热菌蛋白酶切割蛋白质后获得的多肽的自动Edman降解。所提出的蛋白质可变区的序列表明，它可能属于V λ I亚组。胰蛋白酶和嗜热菌蛋白酶的肽都含有碳水化合物的分离和表征，并建立连接到天冬酰胺的寡糖链的本地化。
The amino-acid sequence of the variable region of a carbohydrate-containing amyloid fibril protein EPS of Ig .lambda. light chain origin was elucidated. The protein was isolated from the liver of a patient (EPS) with an immunocyte dyscrasia of the IgM type. The molecular mass of this protein was found to be about 20 kDa [Kdalton] including an oligosaccharide chain linked to it. The amino-acid sequence determination involved automatic Edman degradation of polypeptides obtained after cleaving the protein with BNPS-skatole, trypsin and thermolysin. The proposed sequence of the variable region of the protein showed that it may be assigned to the V.lambda.I subgroup. A tryptic and a thermolysinolytic peptide both containing the carbohydrate were isolated and characterized, and the localization of an oligosaccharide chain linked to asparagine was established.