THE MOUSE PROTEIN-SYNTHESIS INITIATION FACTOR-4A GENE FAMILY INCLUDES 2 RELATED FUNCTIONAL GENES WHICH ARE DIFFERENTIALLY EXPRESSED
THE MOUSE PROTEIN-SYNTHESIS INITIATION FACTOR-4A GENE FAMILY INCLUDES 2 RELATED FUNCTIONAL GENES WHICH ARE DIFFERENTIALLY EXPRESSED
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DOI：
10.1002/j.1460-2075.1988.tb03049.x
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发表时间：
1988-07-01
期刊：
影响因子：
11.4
通讯作者：
TRACHSEL, H
中科院分区：
文献类型：
--
作者：
NIELSEN, PJ;TRACHSEL, H
我们克隆并鉴定了与编码 eIF-4A 的小鼠 cDNA 探针杂交的小鼠基因组序列家族，eIF-4A 是参与 mRNA 与核糖体结合的蛋白质合成起始因子之一。我们估计总共有.apprx。 9-13 eIF-4A 假基因。我们还发现了一个 eIF-4A 无内含子反座子，与 cDNA 相比，它包含单核苷酸差异。这个可能有功能的基因包含整合在启动子区域的小鼠重复 B1 元件。此外，我们克隆了两个含有内含子的 eIF-4A 基因（称为 eIF-4AI 和 eIF-4AII）。 eIF-4AII 基因编码以前未知形式的 eIF-4A。与来自多个小鼠器官的 RNA 进行的 Northern 印迹杂交显示，eIF-4AI 表达的差异在 7 倍以内。相比之下，相对于肝脏，eIF-4AII 表达在脑和肾中高 20 至 30 倍，在肺和心脏中高 10 至 17 倍，并且在肝、脾和胸腺中的表达量大致相同。这些数据表明，不同 mRNA 的蛋白质合成起始相对效率（如信使 5'' 末端帽识别和与核糖体结合的区别所反映）在不同组织中有所不同。
We have cloned and characterized a family of mouse genomic sequences hybridizing to mouse cDNA probes coding for eIF-4A, one of the protein synthesis initiation factors involved in the binding of mRNA to the ribosome. We estimate that there is a total of .apprx. 9-13 eIF-4A pseudogenes. We also found an eIF-4A intronless retroposon which, when compared to the cDNA, contains a single nucleotide difference. This possibly functional gene contains a mouse repetitive B1 element integrated in the promoter region. Furthermore, we have cloned two intron-containing eIF-4A genes (termed eIF-4AI and eIF-4AII). The eIF-4AII gene codes for a previously unknown form of eIF-4A. Northern blot hybridization with RNA from several mouse organs shows a variation in eIF-4AI expression within a factor of 7. In contrast, relative to liver, eIF-4AII expression is 20- to 30-times higher in brain and kidney, 10- to 17-fold higher in lung and heart, and is about equally abundant in liver, spleen and thymus. These data suggest that the relative efficiency of protein synthesis initiation for different mRNAs, as reflected by discrimination in messenger 5''-terminal cap recognition and binding to ribosomes, varies in different tissues.