REGIONAL γ‐AMINOBUTYRIC ACID LEVELS IN RAT BRAIN DETERMINED AFTER MICROWAVE FIXATION
REGIONAL γ‐AMINOBUTYRIC ACID LEVELS IN RAT BRAIN DETERMINED AFTER MICROWAVE FIXATION
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微波固定后测定大鼠脑内区域 γ-氨基丁酸水平
DOI：
10.1111/j.1471-4159.1975.tb03835.x
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发表时间：
1975
影响因子：
4.7
通讯作者：
J. Meyerhoff
中科院分区：
文献类型：
--
作者：
G. J. Balcom;R. Lenox;J. Meyerhoff
—通过三种处死方法比较大鼠全脑中的 GABA 水平：快速微波固定、液氮中断头和 20°C 下断头。通过微波固定和斩首进入液氮而处死的动物中，其水平是相同的。相比之下，在 20°C 下斩首的大鼠在死后立即测定其 GABA 水平高出 18%，在 20°C 下 30 分钟后水平高出 48%。微波处理阻止了这些死后的增加。 20°C 断头后，下丘脑中 GABA 的增加甚至比整个大脑中的还要多。
—GABA levels in rat whole brain were compared following three methods of sacrifice: rapid microwave fixation, decapitation into liquid nitrogen, and decapitation at 20°C. Levels were shown to be identical in animals sacrificed by microwave fixation and decapitation into liquid nitrogen. In contrast, rats decapitated at 20°C had 18 per cent higher GABA levels when determined immediately post‐mortem and 48 per cent higher levels after 30 min at 20°C. Microwave treatment prevented these post‐mortem increases. The increase in GABA after decapitation at 20°C was even greater in hypothalamus than in whole brain.