Expression of 300-kilodalton intermediate filament-associated protein distinguishes human glioma cells from normal astrocytes.
Expression of 300-kilodalton intermediate filament-associated protein distinguishes human glioma cells from normal astrocytes.
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300 千道尔顿中间丝相关蛋白的表达可将人类神经胶质瘤细胞与正常星形胶质细胞区分开来。
DOI：
10.1073/pnas.90.18.8534
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发表时间：
1993
影响因子：
11.1
通讯作者：
Pappas,GD
中科院分区：
文献类型：
--
作者：
Yang,HY;Lieska,N;Glick,R;Shao,D;Pappas,GD
利用生化标记物来区分胶质瘤细胞和正常星形胶质细胞将具有巨大的诊断价值。这些标记物在研究人类星形细胞瘤的基础生物学方面也可能有价值。波形蛋白结合，300 kDa的中间丝（IF）相关蛋白（IFAP-300 kDa）最近已被证明是在大鼠中枢神经系统的放射状胶质细胞发育表达。在成年大鼠的正常或反应性星形胶质细胞中未检测到，也未在原代培养的新生大鼠脑星形胶质细胞中检测到。在本研究中，使用IFAP-300 kDa和胶质细胞酸性蛋白（GFAP，星形胶质细胞特异性IF结构蛋白）的抗体的双标记免疫荧光显微镜从所有三种主要类型的人类星形胶质细胞瘤（即，分化良好、间变性和多形性胶质母细胞瘤）。从所有三种星形细胞瘤制备的原代培养物中的星形细胞瘤细胞也表达这种蛋白质。在正常成人脑组织中是检测不到的。使用IFAP-300 kDa抗体的免疫印迹分析证实了在富含IF蛋白的新鲜星形细胞瘤制剂中存在300-kDa多肽。这些结果表明IFAP-300 kDa可作为体外和原位鉴定人脑胶质瘤细胞的标志物。
The availability of biochemical markers to distinguish glioma cells from normal astrocytes would have enormous diagnostic value. Such markers also may be of value in studying the basic biology of human astrocytomas. The vimentin-binding, 300-kDa intermediate filament (IF)-associated protein (IFAP-300kDa) has recently been shown to be developmentally expressed in radial glia of the central nervous system of the rat. It is not detected in the normal or reactive astrocytes of the adult rat nor in neonatal rat brain astrocytes in primary culture. In the present study, double-label immunofluorescence microscopy using antibodies to IFAP-300kDa and glial fibrillary acidic protein (GFAP, an astrocyte-specific IF structural protein) identifies this IFAP in GFAP-containing tumor cells from examples of all three major types of human astrocytomas (i.e., well-differentiated, anaplastic, and glioblastoma multiforme). Astrocytoma cells in primary cultures prepared from all three astrocytomas also express this protein. It is not detectable in normal adult brain tissue. Immunoblot analyses using the IFAP-300kDa antibody confirm the presence of a 300-kDa polypeptide in fresh astrocytoma preparations enriched for IF proteins. These results suggest the utility of IFAP-300kDa as a marker for identification of human glioma cells both in vitro and in situ.