Removal of ammonium in surface water at low temperature by a newly isolated Microbacterium sp. strain SFA13.
Removal of ammonium in surface water at low temperature by a newly isolated Microbacterium sp. strain SFA13.
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DOI：
10.1016/j.biortech.2013.03.094
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发表时间：
2013-06
影响因子：
11.4
通讯作者：
Duoying Zhang;Weiguang Li;Xiaofei Huang;W. Qin;Miao Liu
中科院分区：
文献类型：
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作者：
Duoying Zhang;Weiguang Li;Xiaofei Huang;W. Qin;Miao Liu
从松花江中分离得到的菌株SFA 13在低温好氧条件下具有将氨氮转化为氮的能力。根据16 S rRNA基因序列分析，确定该菌株为微杆菌属的一个种。该菌株具有较强的自养硝化能力，在5°C时的硝化速率为0.11mgNH4 +-N/L/h。菌株SFA 13在降解有机碳的同时消耗铵态氮，不积累亚硝酸盐和硝酸盐。NO3−-N或NO2−-N被菌株SFA 13还原。反硝化率为0.24mgNO3−-N/L/h。羟胺氧化酶、亚硝酸盐还原酶和硝酸盐还原酶可检测到。该菌株的脱氮过程为：NH 4 +→ NH 2 OH →NO2−→NO3−，然后NO3−→NO2−→N2。在C/N为2-10、温度为10°C、DO>5.2mg/L的条件下，生物活性炭对氨氮的去除率为2.68±0.27-3.16±0.25mgNH4+-N/L/h。
The strain SFA13, isolated from Songhua River, demonstrates ability to convert ammonium to nitrogen under aerobic conditions at low temperature. On the basis of 16S rRNA gene sequence, the strain SFA13 was a species in genera Microbacterium. The isolate showed unusual ability of autotrophic nitrification with the ratio of 0.11mg NH4+-N/L/h at 5°C. Ammonium was consumed by the strain SFA13 with the biodegradation of organic carbon and without nitrite or nitrate accumulation. NO3−-N or NO2−-N was reduced by the strain SFA13. The denitrification ratio was 0.24mgNO3−-N/L/h. Hydroxylamine oxidase, nitrite reductase and nitrate reductase were detectable. The putative nitrogen removal process by the strain SFA13 was as follows: NH4+→NH2OH→NO2−→NO3−, then NO3−→NO2−→N2. Biological activated carbon attached with the strain SFA13 could effectively remove ammonium in surface water with the rate of 2.68±0.27–3.16±0.25mgNH4+-N/L/h at C/N 2–10, temperature 10°C, and DO>5.2mg/L.