BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Reactive blue 19 decolouration by laccase immobilized on silica beads
BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Reactive blue 19 decolouration by laccase immobilized on silica beads
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发表时间：
2007
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通讯作者：
Paul-Philippe Champagne;J. Ramsay
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作者：
Paul-Philippe Champagne;J. Ramsay
将杂色栓菌（Trametesversicolor）的漆酶（31.5U/g或4.39 μg蛋白/m2）固定在多孔载体硅胶珠上，并对蒽醌染料活性蓝19进行脱色。虽然有一个初始的，快速吸附的染料的填充床在循环反应器中，约97.5%的活性蓝19去除是由于酶降解。游离酶在48 h内失去52%的活性。然而，固定化漆酶的活性不变后，在磷酸盐缓冲液中在环境条件下储存4个月，随后在120小时内连续三次脱色。用乙醇胺处理漆酶固定化小球，染料吸附量降低40%。
Laccase (31.5 U of activity/g or 4.39 μg of protein/m) from Trametes versicolor was immobilized on controlled-porosity-carrier silica beads and evaluated for the decolouration of Reactive blue 19, an anthraquinone dye. Although there was an initial, rapid adsorption of the dye to the packed bed in a recirculating reactor, about 97.5% of Reactive blue 19 removal was due to enzymatic degradation. The free enzyme lost 52% of its activity in 48 h. However, the activity of the immobilized laccase was unchanged after 4 months of storage in phosphate buffer under ambient conditions followed by three successive decolourations over 120 h. Treating the laccase immobilized beads with ethanolamine reduced dye adsorption by 40%.