First isolation and characterization of Getah virus from cattle in northeastern China
First isolation and characterization of Getah virus from cattle in northeastern China
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首次从中国东北地区的牛中分离和鉴定 Getah 病毒。
DOI：
10.1186/s12917-019-2061-z
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发表时间：
2019-09-05
影响因子：
2.6
通讯作者：
Si, Xing-kui
中科院分区：
文献类型：
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作者：
Liu, Hao;Zhang, Xu;Si, Xing-kui
盖塔病毒(Getah Virus，GETV)是一种被忽视的蚊媒甲型病毒，可引起马发热、皮疹和腿部水肿，并可导致猪胎儿死亡和生殖障碍。受感染的动物可能在病毒的放大和传播中发挥关键作用。本研究旨在调查中国东北地区临床感染的牛和媒介蚊种中的GETV感染情况。结果采集了森林牧区突然发病的肉牛的血清样本，并进行了基因组测序，从10份血清样本中发现了29个与GETV基因组匹配的重叠群。对48份肉牛血清样品进行了GETV RNA定量RT-PCR(RT-qPCR)，结果表明，肉牛血清中GETV的总感染率为6.25%(3/48)。血清学调查显示，研究区83.3%(40/48，95%可信区间67~100)的样本检出了GETV中和抗体。GETV JL1808株是从临床感染的表现发热的牛身上分离出来的。序列比较显示，与2017年在湖南省获得的高致病性猪瘟分离株HUN1毒株在核苷酸水平(99.5%)和推导的氨基酸水平(99.7%-99.9%)上有很高的同源性。对JL1808毒株的系统发育分析表明，JL1808毒株与HUN1毒株有较近的亲缘关系。此外，约有12000只蚊子被困在这一地区。在蚊体中检出GETV感染，其最小感染率为1.50，其中三带喙库蚊为1.67‰，三带喙库蚊为1.60‰，中华按蚊为1.21‰。这些结果表明，一种高致病性的蚊媒猪瘟病毒可以在牛体内感染和传播，这表明有必要对中国东北部的动物进行猪瘟病毒感染的监测。
BackgroundGetah virus (GETV) is a neglected mosquito-borne Alphavirus that causes pyrexia, body rash, and leg oedema in horses and foetal death and reproductive disorders in pigs. Infected animals may play a critical role in the amplification and circulation of the virus. The present study aimed to investigate GETV infection in clinically infected cattle and vector mosquito species in northeastern China.ResultsSerum samples were collected from beef cattle that presented sudden onset of fever in forest grazing areas, and metagenomic sequencing was conducted, revealing 29 contigs from ten serum samples matching the GETV genome. Quantitative RT-PCR (RT-qPCR) was performed with GETV RNA from 48 beef cattle serum samples, showing that the overall prevalence of GETV in the beef cattle samples was 6.25% (3/48). Serological investigation indicated that GETV neutralizing antibodies were detected in 83.3% (40/48, 95% CI 67-100) of samples from the study region. The GETV JL1808 strain was isolated from clinically infected cattle showing fever. Sequence comparisons showed high identity with the HuN1 strain, a highly pathogenic swine epidemic isolate obtained in Hunan province in 2017, at the nucleotide level (99.5%) and at the deduced amino acid level (99.7-99.9%). The phylogenetic analysis of JL1808 clustered in Group III, and also revealed a close genetic relationship with the HuN1 strain. Additionally, about 12,000 mosquitoes were trapped in this region. The presence of GETV infection was detected in mosquitoes, suggesting that the minimum infection rate (MIR) was 1.50, with MIRs of 1.67 parts per thousand in Culex pseudovishnui, 1.60 parts per thousand in Culex tritaeniorhynchus, and 1.21 parts per thousand in Anopheles sinensis.Conclusions p id=Par To the best of our knowledge, this is the first report of GETV infection in cattle. These results demonstrated that a highly pathogenic, mosquito-borne swine GETV can infect and circulate in cattle, implying that it is necessary to conduct surveillance of GETV infection in animals in northeastern China.