Marked changes in volume of mesophyll protoplasts of pea (Pisum sativum) on exposure to growth hormones.
Marked changes in volume of mesophyll protoplasts of pea (Pisum sativum) on exposure to growth hormones.
复制标题
DOI：
10.1078/0176-1617-01076
复制
发表时间：
2004
影响因子：
4.3
通讯作者：
V. Kolla;Dontamala Suhita;A. S. Raghavendra
中科院分区：
文献类型：
--
作者：
V. Kolla;Dontamala Suhita;A. S. Raghavendra
本研究报告了植物激素诱导豌豆叶肉原生质体体积的快速和显着的变化。四种植物激素：赤霉酸（GA_3）、吲哚乙酸（IAA）、脱落酸（阿坝）（+/-）和茉莉酸甲酯（MJ）引起叶肉原生质体体积的显著变化。GA 3和IAA增加了原生质体的体积（高达90%），而阿坝和MJ减少了体积（约40%）。水通道蛋白或水通道似乎在原生质体的膨胀/收缩中起重要作用，如由HgCl 2抑制体积变化和巯基乙醇逆转所示。第二信使的可能作用，在体积变化引起的GA 3进行了研究，通过使用选定的药理学试剂。GDP-beta-S（G蛋白拮抗剂）、U 73122（磷脂酶C抑制剂）和TFP（钙调蛋白拮抗剂）可抑制GA 3诱导的肿胀，但1-丁醇（磷脂酶D抑制剂）、GTP-gamma-S（G蛋白激动剂）或维拉帕米（钙通道阻滞剂）对GA 3诱导的肿胀无影响。结果表明，叶肉原生质体是一种简单而有用的研究植物组织体积变化的系统。
The present study reports quick and significant changes induced by plant hormones in the volume of mesophyll protoplasts of pea (Pisum sativum). Four plant hormones: gibberellic acid (GA3), indole 3-acetic acid (IAA), abscisic acid (ABA)(+/-) and methyl jasmonate (MJ), caused marked changes in the volume of mesophyll protoplasts. GA3 and IAA increased the volume of the protoplasts (up to 90%) whereas the ABA and MJ decreased (by about 40%) the volume. Aquaporins or water channels appear to play an important role in swelling/shrinkage of the protoplasts as indicated by the suppression of volume changes by HgCl2 and reversal by mercaptoethanol. The possible role of secondary messengers in volume changes induced by GA3 was investigated by using selected pharmacological reagents. The GA3 induced swelling was restricted by GDP-beta-S (G-protein antagonist), U73122 (phospholipase C inhibitor), and TFP (calmodulin antagonist), but was not affected by 1-butanol (phospholipase D inhibitor), GTP-gamma-S (G-protein agonist), or verapamil (calcium channel blocker). The results suggest that the mesophyll protoplasts can be a simple and useful system for further studies on volume changes in plant tissues.