Restriction mapping in nanofluidic devices
Restriction mapping in nanofluidic devices
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DOI：
10.1073/pnas.0503809102
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发表时间：
2005-07-19
影响因子：
11.1
通讯作者：
Austin, RH
中科院分区：
文献类型：
--
作者：
Riehn, R;Lu, MC;Austin, RH
我们已经在直径为100-200 rim的纳米通道中使用限制性内切核酸酶进行了DNA分子的限制性作图。通过电泳和Mg 2+和EDTA的扩散控制装置内限制性反应的位置。我们已经成功地使用了限制性内切酶Smal、Sad和Pacl，并且已经能够使用单个DNA分子在1分钟内以约1.5kbp的精度测量限制性位点的位置。
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 rim. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes Smal, Sad, and Pacl, and have been able to measure the positions of restriction sites with a precision of approximate to 1.5 kbp in 1 min using single DNA molecules.