Thyroid cancer cell lines: an overview.
Thyroid cancer cell lines: an overview.
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甲状腺癌细胞系：概述。
DOI：
10.3389/fendo.2012.00133
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发表时间：
2012
影响因子：
5.2
通讯作者：
Maenhaut C
中科院分区：
文献类型：
--
作者：
Saiselet M;Floor S;Tarabichi M;Dom G;Hébrant A;van Staveren WC;Maenhaut C
人甲状腺癌细胞系是甲状腺癌研究中最常用的模型。必须在详细了解其特性的情况下使用它们。这些体外细胞系来源于体内分化和去分化的人甲状腺肿瘤。然而，它已被证明，这些细胞系的mRNA表达谱更接近去分化体内甲状腺肿瘤（间变性甲状腺癌，ATC）比分化的。在这里，这些模型的知识进行了概述。6个人甲状腺癌细胞系（WRO、FTC 133、BCPAP、TPC 1、K1和8505 C）的突变状态与先前报道的甲状腺癌中10个基因频繁突变的结果一致。然而，WRO中BRAF突变（T1799 A：V600 E）的存在对该细胞系作为滤泡性甲状腺癌（FTC）模型的使用提出了质疑。接下来，为了研究这些细胞中调节的mRNA的生物学意义，对先前获得的mRNA谱进行了5个细胞系的途径分析。在5个细胞系中，MHC II类途径下调，其中4个，核糖体生物合成和翻译途径上调。还将细胞系的mRNA表达谱与不同类型的甲状腺癌的mRNA表达谱进行了比较。使用了源自不同微阵列平台和来自不同实验室的三个数据集。该荟萃分析显示，甲状腺癌细胞系和ATC的特征之间的相关性显著高于分化型甲状腺肿瘤（即，PTC或FTC），专门用于DNA复制。这种已经观察到的更高的相关性是在体内肿瘤数量增加和使用不同平台的情况下获得的。总之，这表明一些乳头状甲状腺癌或滤泡状甲状腺癌（PTC或FTC）细胞系（即，TPC-1）在体外细胞适应/进化过程中可能部分丧失了其原有的DNA合成/复制调控机制。
Human thyroid cancer cell lines are the most used models for thyroid cancer studies. They must be used with detailed knowledge of their characteristics. These in vitro cell lines originate from differentiated and dedifferentiated in vivo human thyroid tumors. However, it has been shown that mRNA expression profiles of these cell lines were closer to dedifferentiated in vivo thyroid tumors (anaplastic thyroid carcinoma, ATC) than to differentiated ones. Here an overview of the knowledge of these models was made. The mutational status of six human thyroid cancer cell lines (WRO, FTC133, BCPAP, TPC1, K1, and 8505C) was in line with previously reported findings for 10 genes frequently mutated in thyroid cancer. However, the presence of a BRAF mutation (T1799A: V600E) in WRO questions the use of this cell line as a model for follicular thyroid carcinoma (FTC). Next, to investigate the biological meaning of the modulated mRNAs in these cells, a pathway analysis on previously obtained mRNA profiles was performed on five cell lines. In five cell lines, the MHC class II pathway was down-regulated and in four of them, ribosome biosynthesis and translation pathways were up-regulated. mRNA expression profiles of the cell lines were also compared to those of the different types of thyroid cancers. Three datasets originating from different microarray platforms and derived from distinct laboratories were used. This meta-analysis showed a significant higher correlation between the profiles of the thyroid cancer cell lines and ATC, than to differentiated thyroid tumors (i.e., PTC or FTC) specifically for DNA replication. This already observed higher correlation was obtained here with an increased number of in vivo tumors and using different platforms. In summary, this would suggest that some papillary thyroid carcinoma or follicular thyroid carcinoma (PTC or FTC) cell lines (i.e., TPC-1) might have partially lost their original DNA synthesis/replication regulation mechanisms during their in vitro cell adaptation/evolution.