Diamidino yellow dihydrochloride (DY·2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell
Diamidino yellow dihydrochloride (DY·2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell
复制标题
二脒黄二盐酸盐 (DY·2HCl)；一种新型荧光逆行神经元示踪剂，只能非常缓慢地迁移出细胞
作者：
Dr. K. Keizer;H. Kuypers;A. M. Huisman;O. Dann
早期的研究表明，核黄（NY）、真蓝（TB）和快蓝（FB）是通过轴突逆行转运到母细胞体的。NY在360 nm激发波长下产生神经元核的黄色荧光标记，而TB和FB在同一波长下产生细胞质的蓝色荧光。因此，NY可能与TB或FB联合进行双标记实验，证明轴突侧枝的存在。然而，逆行标记TB或FB的神经元需要相对较长的生存时间，而NY需要较短的生存时间，因为NY从逆行标记的神经元中迅速迁移。这使双重标记实验变得复杂，因为必须先注射TB和FB，然后再注射NY，在动物被牺牲之前很短的时间。我们报告了一种新的黄色荧光示踪剂，它主要标记细胞核，并且从逆行标记的神经元中迁移的速度比NY慢得多。该示踪剂可代替NY与TB或FB联合用于双标记实验，与NY不同，可与TB或FB同时注射。新的示踪剂是一种二氨基化合物(no。28826)，由Dr. Illing KG商业分发，Warthweg 14-18, Postfach 1150, D-6114 Groß-Umstadt， FRG可用。将其称为二氨基黄盐酸盐（DY·2HCl）。根据本研究，DY·2HCl在大鼠和猫体内长距离运输，并在360 nm激发波长下产生神经元核的黄色荧光，类似于NY获得的荧光。当与TB或FB联合使用时，DY·2HCl通过轴突侧枝发散的方式对神经元进行双重标记的效果与NY相同。
SummaryEarlier studies showed that Nuclear Yellow (NY), True Blue (TB) and Fast Blue (FB) are transported retrogradely through axons to their parent cell bodies. NY produces a yellow fluorescent labeling of the neuronal nucleus at 360 nm excitation wavelength, while TB and FB produce a blue fluorescence of the cytoplasm at this same wavelength. Therefore, NY may be combined with TB or FB in double-labeling experiments demonstrating the existence of axon collaterals. However, retrograde neuronal labeling with TB or FB requires a relatively long survival time, while NY requires a short survival time since NY migrates rapidly out of the retrogradely labeled neurons. This complicates double-labeling experiments since TB and FB must be injected first and NY later, a short time before the animal is sacrificed. We report a new yellow fluorescent tracer which labels mainly the nucleus and migrates much more slowly out of the retrogradely labeled neurons than NY. This new tracer can be used instead of NY in combination with TB or FB in double-labeling experiments and unlike NY can be injected at the same time as TB or FB. The new tracer is a diamidino compound (no. 28826) which is commerciallyDistributed by Dr. Illing KG, Warthweg 14-18, Postfach 1150, D-6114 Groß-Umstadt, FRG available. It will be referred to as Diamidino Yellow Dihydrochloride (DY·2HCl). According to the present study DY·2HCl is transported over long distances in rat and cat, and produces a yellow fluorescence of the neuronal nucleus at 360 nm excitation wavelength, resembling that obtained with NY. When combined with TB or FB, DY·2HCl is as effective as NY in double labeling of neurons by way of divergent axon collaterals.