LONG-TERM EXPRESSION OF HUMAN ADENOSINE-DEAMINASE IN VASCULAR SMOOTH-MUSCLE CELLS OF RATS - A MODEL FOR GENE-THERAPY
LONG-TERM EXPRESSION OF HUMAN ADENOSINE-DEAMINASE IN VASCULAR SMOOTH-MUSCLE CELLS OF RATS - A MODEL FOR GENE-THERAPY
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DOI：
10.1073/pnas.89.3.1138
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发表时间：
1992-02-01
影响因子：
11.1
通讯作者：
MILLER, AD
中科院分区：
文献类型：
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作者：
LYNCH, CM;CLOWES, MM;MILLER, AD
用含有大肠杆菌β-半乳糖苷酶基因或人腺苷脱氨酶基因的重组逆转录病毒载体将基因转移到动物血管平滑肌细胞中。未观察到通过向大鼠颈动脉注入病毒的直接基因转移。然而，通过在培养中感染血管平滑肌细胞并将转导细胞种植到已剥离内皮细胞的动脉上进行基因转移是成功的。在6个月的观察中，检测到了潜在的治疗水平的人腺苷脱氨酶活性，表明血管平滑肌细胞在人类基因治疗中的应用。
Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.