Changes of colonic mucosal microcirculation and histology in two colitis models -: An experimental study using intravital microscopy and a new histological scoring system
Changes of colonic mucosal microcirculation and histology in two colitis models -: An experimental study using intravital microscopy and a new histological scoring system
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DOI：
10.1023/a:1012334727509
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发表时间：
2001-11-01
影响因子：
3.1
通讯作者：
Buhr, HJ
中科院分区：
文献类型：
--
作者：
Kruschewski, M;Foitzik, T;Buhr, HJ
本研究探讨了三硝基苯磺酸（TNBS）或丝裂霉素C诱导的大鼠结肠炎发病后不同时间不同肠段粘膜的毛细血管血流量（CBF）和病理形态学变化。使用荧光素标记的红细胞通过活体显微镜测定CBF。通过一种新的评分系统评估炎症的组织学程度。在诱导TNBS结肠炎后24小时，在远端结肠中发现严重的急性组织学变化（评分：8.9 +/-1.0）。CBF增加（2.9 +/- 0.05 vs.健康对照组2.6 +/- 0.04 nl/min）。组织学变化持续到第3天（8.5 +/- 0.9），此时CBF显著降低（1.8 +/- 0.05 nl/min）。15天后，组织学上仍可检测到中度急性炎症（5.4 ± 1.3），但CBF已恢复正常值。在丝裂霉素C结肠炎中，变化主要发生在近端结肠：3天后，出现轻度炎症（2.8 ± 1.2），CBF正常（2.5 ± 0.1 nl/min）。7天后，炎症增加（4.8 +/- 1.1），而CBF减少（1.5 +/- 0.06 nl/min）。这些变化持续6周（5.3 ± 0.7; 1.2 ± 0.05 nl/min）。这些数据表明，结肠微循环障碍可能在炎症性肠病的发病机制中发挥重要作用，无论组织形态学改变。
This study investigated capillary blood flow (CBF) and pathomorphological alterations in the mucosa of different bowel segments at different times after disease onset in rats with colitis induced by either trinitrobenzensulfonic acid (TNBS) or mitomycin-C. CBF was determined by intravital microscopy using fluorescein-labeled erythrocytes. The histological degree of inflammation was assessed by a new scoring system. Severe acute histological changes were found in the distal colon 24 hr after induction of TNBS colitis (score: 8.9 +/- 1.0). CBF was increased (2.9 +/- 0.05 vs. 2.6 +/- 0.04 nl/min in healthy controls). The histological alterations persisted until day 3 (8.5 +/- 0.9) when CBF significantly decreased (1.8 +/- 0.05 nl/min). After 15 days, moderate acute inflammation was still detectable histologically (5.4 +/- 1.3), but CBF had returned to normal values. In mitomycin-C colitis, changes developed mainly in the proximal colon: After three days, there was mild inflammation (2.8 +/- 1.2) with normal CBF (2.5 +/- 0.1 nl/min). After seven days, the inflammation had increased (4.8 +/- 1.1), while CBF had decreased (1.5 +/- 0.06 nl/min). These changes persisted for six weeks (5.3 +/- 0.7; 1.2 +/- 0.05 nl/min). These data suggest that disturbed colonic microcirculation may play an important role in the pathogenesis of inflammatory bowel disease regardless of the histopathomorphological alterations.