Quantitative trait loci for yield and yield components in an Oryza sativa x Oryza rufipogon BC2F2 population evaluated in an upland environment
Quantitative trait loci for yield and yield components in an Oryza sativa x Oryza rufipogon BC2F2 population evaluated in an upland environment
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DOI：
10.1007/s001220051616
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
McCouch, SR
中科院分区：
文献类型：
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作者：
Moncada, P;Martínez, CP;McCouch, SR
采用先进的回交育种策略，对旱稻亚种Caiapo的BC2F2群体中8个农艺性状相关的数量性状位点（qtl）进行了鉴定。来自巴西的粳稻品种，以及来自马来西亚的稻新品种。Caiapo是拉丁美洲种植最广泛的旱地栽培品种之一，可作为单一栽培或与牧场混合种植。本研究的目的是：(1)确定在Caiapo所适应的干旱易发酸性土壤条件下生长的274个BC2F2家族中是否会检测到来自O. rufipogon的性状增强qtl；(2)比较在有牧场竞争和没有牧场竞争的情况下的表现；(3)将本研究确定的假定含有qtl的区域与先前报道的或适应灌溉低地条件的种群进行比较。通过对分布在全基因组中的125个SSLP和RFLP标记的分析，利用单点定位、区间定位和复合区间定位，共检测到2个与产量有关的qtl， 13个与产量成分有关，4个与成熟度有关，6个与株高有关。我们认为，先进的回交QTL分析为适应逆境环境的品种的遗传改良提供了一种有用的种质增强策略。虽然野生种质的表型表现并不表明其作为育种亲本的价值，但值得注意的是，本研究中鉴定的56%的性状增强qtl来自O. rufipogon。这一数字与先前研究中在灌溉条件下评估的高产杂交稻品种杂交中来自同一亲本的有利qtl的51%相似。总之，利用AB-QTL分析对水稻进行的平行研究提供了越来越多的证据，表明水稻基因组的某些区域可能含有多种环境下植物改良的相关基因。
An advanced backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with eight agronomic traits in a BC2F2 population derived from an interspecific cross between Caiapo, an upland Oryza sativa subsp. japonica rice variety from Brazil, and an accession of Oryza rufipogon from Malaysia. Caiapo is one of the most-widely grown dryland cultivars in Latin America and may be planted as a monoculture or in a multicropping system with pastures. The objectives of this study were: (1) to determine whether trait-enhancing QTLs from O. rufipogon would be detected in 274 BC2F2 families grown under the drought prone, acid soil conditions to which Caiapo was adapted, (2) to compare the performance with and without pasture competition, and (3) to compare putative QTL-containing regions identified in this study with those previously reported or populations adapted to irrigated, low-land conditions. Based on analyses of 125 SSLP and RFLP markers distributed throughout the genome and using single-point, interval, and composite interval mapping, two putative O. rufipogon derived QTLs were detected for yield, 13 for yield components, four for maturity and six for plant height. We conclude that advanced backcross QTL analysis offers a useful germplasm enhancement strategy for the genetic improvement of cultivars adapted to stress-prone environments. Although the phenotypic performance of the wild germplasm would not suggest its value as a breeding parent, it is noteworthy that 56% of the trait-enhancing QTLs identified in this study were derived from O. rufipogon. This figure is similar to the 51% of favorable QTLs derived from the same parent in crosses with a high-yielding hybrid rice cultivar evaluated under irrigated conditions in a previous study. In conclusion, parallel studies in rice using AB-QTL analysis provide increasing evidence that certain regions of the rice genome are likely to harbor genes of interest for plant improvement in multiple environments.