Tuning shape and internal structure of protein droplets via biopolymer filaments
Tuning shape and internal structure of protein droplets via biopolymer filaments
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通过生物聚合物丝调整蛋白质液滴的形状和内部结构
DOI：
10.1039/c9sm02462j
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发表时间：
2020
期刊：
影响因子：
3.4
通讯作者：
Gardel, Margaret L.
中科院分区：
文献类型：
--
作者：
Scheff, Danielle R.;Weirich, Kimberly L.;Dasbiswas, Kinjal;Patel, Avinash;Vaikuntanathan, Suriyanarayanan;Gardel, Margaret L.
大分子可以相分离形成液态冷凝物，这在细胞内组织和软材料设计等领域成为关键的隔室。包括蛋白质FUS在内的无数大分子形成凝聚体，表现为各向同性液体。本文研究了长丝掺杂剂对蛋白液体材料性能的影响。我们发现肌动蛋白的短生物聚合物细丝自发地分裂成FUS液滴，形成复合液滴。随着长丝掺杂剂浓度的增加，聚结时间缩短，表明掺杂剂相对于表面张力控制粘度。液滴的形状是可调的，随着长丝长度或浓度的增加，液滴的形状从球形到液滴状不等。我们发现，准双极液晶液滴的模型很好地描述了液滴形状，其中各向异性肌动蛋白丝的向列顺序与来自FUS的各向同性界面能竞争，以尺寸依赖的方式控制液滴形状。我们的研究结果展示了一种构建可调、各向异性大分子液体的通用方法。
Macromolecules can phase separate to form liquid condensates, which are emerging as critical compartments in fields as diverse as intracellular organization and soft materials design. A myriad of macromolecules, including the protein FUS, form condensates which behave as isotropic liquids. Here, we investigate the influence of filament dopants on the material properties of protein liquids. We find that the short, biopolymer filaments of actin spontaneously partition into FUS droplets to form composite liquid droplets. As the concentration of the filament dopants increases, the coalescence time decreases, indicating that the dopants control viscosity relative to surface tension. The droplet shape is tunable and ranges from spherical to tactoid as the filament length or concentration is increased. We find that the tactoids are well described by a model of a quasi bipolar liquid crystal droplet, where nematic order from the anisotropic actin filaments competes with isotropic interfacial energy from the FUS, controlling droplet shape in a size-dependent manner. Our results demonstrate a versatile approach to construct tunable, anisotropic macromolecular liquids.
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影响因子：
18.2
作者：
Lytle, Tyler K.;Chang, Li-Wei;Sing, Charles E.
通讯作者：
Sing, Charles E.
F-肌动蛋白加帽蛋白。
影响因子：
7.5
作者：
A. Weeds;SutherlandK. Maciver
通讯作者：
SutherlandK. Maciver
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
DOI：
10.1073/pnas.1409404111
发表时间：
2014-12-30
影响因子：
11.1
作者：
Brugues, Jan;Needleman, Daniel
通讯作者：
Needleman, Daniel
影响因子：
15.6
作者：
Marciel AB;Chung EJ;Brettmann BK;Leon L
通讯作者：
Leon L