In vitro evaluation of tipburn resistance in lettuce (Lactuca sativa. L)
In vitro evaluation of tipburn resistance in lettuce (Lactuca sativa. L)
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DOI：
10.1007/s11240-011-0033-5
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发表时间：
2012-02
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影响因子：
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通讯作者：
Ryohei Koyama;Mitsuhiro Sanada;H. Itoh;M. Kanechi;N. Inagaki;Y. Uno
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文献类型：
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作者：
Ryohei Koyama;Mitsuhiro Sanada;H. Itoh;M. Kanechi;N. Inagaki;Y. Uno
生菜尖烧是一种由缺钙引起的不可逆的生理疾病，会降低作物价值。在许多情况下，培育抗尖烧品种是唯一的因果疗法。在这项研究中，我们研究了一种有效的方法来评估生菜对尖烧的体外抗性。代表三种头型的 19 个生菜品种的幼苗在含有 EGTA 的琼脂培养基上培养，EGTA 可螯合 Ca2+。叶尖烧伤的百分比随 EGTA 浓度成比例下降。敏感品种在 0.01 mM EGTA 下被分类，而抗性品种在 1.0 mM EGTA 下被分类。根据尖端烧伤测量的平均值，“叶用生菜”的尖端烧伤敏感性最高，其次是“黄油头生菜”，然后是“脆头生菜”。选择两个品种进行水培和盆栽的进一步测试。这些实验中对尖端烧伤的敏感性的排序与体外测定的一致。生菜对钙缺乏敏感性的体外评估有助于初步筛选生菜品种以预防烧伤发生。本研究中确定的抗性品种是在受控环境（例如植物工厂）中种植的实用候选品种，而敏感品种也可用作监测环境条件的指示植物。
Lettuce tipburn is an irreversible physiological disorder caused by calcium deficiency that decreases the crop value. Breeding a tipburn-resistant cultivar is the only causal therapy in many cases. In this study, we investigated an efficient method to evaluate lettuce resistance to tipburn in vitro. Seedlings of 19 lettuce cultivars representing three head types were cultured on agar medium containing EGTA, which chelates Ca2+. The percentage of tipburned leaves decreased proportionally with EGTA concentration. Susceptible cultivars were distinguished at 0.01 mM EGTA, whereas resistant cultivars were classified at 1.0 mM EGTA. Based on mean values of tipburn measurements, tipburn susceptibility was highest for ‘Leaf Lettuce’, followed by ‘Butterhead Lettuce’, and then ‘Crisphead Lettuce’. Two cultivars were selected for further tests using hydroponic and pot culture. The rank order of susceptibility to tipburn in these experiments was consistent with that of the in vitro assay. The in vitro evaluation of lettuce susceptibility to calcium deficiency is useful for initial screening of lettuce cultivars against tipburn incidence. Resistant cultivars identified in this study are practical candidates for cultivation in controlled environments, such as a plant factory, while sensitive cultivars are also useful as indicator plants to monitor environmental conditions.