Simultaneous detection of multiple DNA targets based on encoding metal ions.
Simultaneous detection of multiple DNA targets based on encoding metal ions.
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DOI：
10.1016/j.bios.2013.09.008
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发表时间：
2014-02
影响因子：
12.6
通讯作者：
Lichun Zheng;Xiaoyan Li;Panpan Liu;Guofan Wu;Xiaoquan Lu;Xiuhui Liu
中科院分区：
文献类型：
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作者：
Lichun Zheng;Xiaoyan Li;Panpan Liu;Guofan Wu;Xiaoquan Lu;Xiuhui Liu
我们提出了一种新的策略，同时电化学检测多个DNA目标的基础上使用不同的编码金属离子作为标签。其原理是金属硫蛋白（MT）分子上结合的金属离子与靶DNA杂交后释放下来，然后用溶出伏安法检测。首次合成了不同金属离子包覆的新型检测探针ssDNA/MT偶联物，并利用3种金属离子（Zn 2+、Cd 2+和Pb 2+）在BiFE上分别在−1.13 V（Zn）、−0.78 V（Cd）和−0.52 V（Pb）处的阳极溶出峰区分3种病毒DNA的信号。在0.1 ~ 10 nM范围内，阳极峰电流与靶DNA浓度呈线性关系，检测限为33 pM。此外，一个碱基错配的目标是有效地区分互补的目标。实验结果表明，该方法具有较高的灵敏度和选择性，可用于多靶点的DNA检测，在生物检测，特别是免疫检测中具有广阔的应用前景。
We present a novel strategy for simultaneous electrochemical detection of multiple DNA targets based on the use of different encoding metal ions as tags. The principle of this scheme is that metal ions bound to metallothionein (MT) molecules can be released down after hybridization with DNA targets and then be detected by stripping voltammetry. The novel detection probes, ssDNA/MT conjugates, covered with different metal ions were synthesized for the first time, then three encoding metal ions (Zn2+, Cd2+, and Pb2+) were used to differentiate the signals of three virus DNA due to their well-defined anodic stripping peaks at −1.13 V (Zn), −0.78 V (Cd), and −0.52 V (Pb) at BiFE, respectively. The anodic peak currents increased linearly with the concentrations of DNA targets in the range from 0.1 nM to 10 nM with a detection limit of 33 pM. In addition, the one-base mismatched target was effectively discriminated from the complementary target. The described results demonstrated that this method possesses high sensitivity and selectivity for multi-target DNA assay and has great potential in applications for detection of even more targets in biological assays, particularly immunoassays.