(1→3)-β-D-glucan and endotoxin in house dust and peak flow variability in children
(1→3)-β-D-glucan and endotoxin in house dust and peak flow variability in children
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DOI：
10.1164/ajrccm.162.4.9909118
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发表时间：
2000-10-01
影响因子：
24.7
通讯作者：
Brunekreef, B
中科院分区：
文献类型：
--
作者：
Douwes, J;Zuidhof, A;Brunekreef, B
与室内灰尘相关的细菌内毒素已被证明与哮喘严重程度相关，真菌（1->3）-β-D-葡聚糖也有类似的作用。在这项研究中，这些药物和呼气峰流速（PEF）的变异性之间的关系进行了调查，在148名儿童7至11岁，其中50%的自我或父母报告的慢性呼吸道症状。所有儿童每天自我监测两次PEF，为期16周。从床垫、起居室和卧室的地板上收集灰尘样本，并在灰尘提取物中测量内毒素和（1->3）-β-D-葡聚糖。通过线性回归分析，调整尘螨过敏原水平，宠物的存在和地板覆盖物类型，研究与平均每日PEF变异性（Ampl%平均值）的关系。在未经校正的分析中，每平方米客厅地板的内毒素和（1->3）-β-D-葡聚糖水平与PEF变异性显著相关（但当以每克采样灰尘表示时则不相关），特别是在有哮喘症状的特应性儿童中。调整后的分析显示，（1->3）-β-D-葡聚糖与内毒素的相关性相同。尽管未发现卧室地板或床垫灰尘中的微生物因子水平相关，但客厅地板灰尘中高水平的（1->3）-β-D-葡聚糖明显增加哮喘儿童的PEF变异性。
House dust-associated bacterial endotoxins have been shown to be associated with asthma severity, and a similar role has been suggested for fungal (1-->3)-beta-D-glucans. In this study the relation between these agents and peak expiratory flow (PEF) variability was investigated in 148 children 7 to 11 yr of age of whom 50% had self- or parent-reported chronic respiratory symptoms. All children self-monitored twice daily their PEF for a period of 16 wk. Dust samples were collected from mattresses and from living room and bedroom floors, and endotoxin and (1-->3)-beta-D-glucan were measured in dust extracts. The relations with mean daily PEF variability (Ampl%mean) were investigated by linear regression analysis, adjusting for dust mite allergen levels, presence of pets, and type of floor cover. In unadjusted analyses the levels of both endotoxin and (1-->3)-beta-D-glucan per square meter of living room floor were significantly associated with PEF-variability (but not when expressed per gram of sampled dust), particularly in atopic children with asthma symptoms. Adjusted analyses showed the same association for (1-->3)-beta-D-glucan but not for endotoxin. Although no associations were found with microbial agent levels in bedroom floor or mattress dust, high levels of (1-->3)-beta-D-glucan in living room floor dust apparently increase PEF variability in asthmatic children.