VELOCITY PROFILES OF BLOOD-PLATELETS AND RED-BLOOD-CELLS FLOWING IN ARTERIOLES OF THE RABBIT MESENTERY
VELOCITY PROFILES OF BLOOD-PLATELETS AND RED-BLOOD-CELLS FLOWING IN ARTERIOLES OF THE RABBIT MESENTERY
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DOI：
10.1161/01.res.59.5.505
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发表时间：
1986-11-01
影响因子：
20.1
通讯作者：
RENEMAN, RS
中科院分区：
文献类型：
--
作者：
TANGELDER, GJ;SLAAF, DW;RENEMAN, RS
在兔肠系膜小动脉（直径17 - 32 μ m）中测定速度分布。通过使用血小板作为流动的小而自然的标记物，获得了良好的空间分辨率，首次在体内提供了关于微血管中的速度分布形状的详细的定量信息。在一些实验中，还记录了红细胞速度分布。可以通过用荧光染料选择性地标记感兴趣的细胞并通过活体荧光视频显微镜使用闪光照明使其可视化来实现感兴趣的细胞的容易检测。两次闪光之间有一个短的预设时间间隔，在一个电视画面中产生同一细胞的两个图像，在给定的时间间隔内移动一定的距离。确定了在血管正中面周围的光学切片内流动的细胞的速度和平均径向位置。血小板和红细胞的速度分布的形状是相似的。与抛物线相比，无论是在收缩期还是收缩期，轮廓都是平坦的。在心动周期内，血管直径没有明显变化。作为轮廓钝化程度的指标，使用轮廓的最大速度与平均速度之比，对于抛物线为2，对于完全活塞流为1。该指数范围为1.39~1.54（中位数1.50），随血管直径增加而增加。计算表明，钝化的配置文件不能解释的影响，有限的深度的光学部分。
Velocity profiles were determined in rabbit mesenteric arterioles (diameter 17-32 .mu.m). A good spatial resolution was obtained by using the blood platelets as small and natural markers of flow, providing for the first time in vivo detailed, quantitative information about the shape of the velocity profiles in microvessels. In some experiments red blood cell velocity profiles were recorded as well. Easy detection of the cells of interest could be achieved by labelling them selectively with a fluorescent dye and visualizing them by intravital fluorescence video microscopy, using flashed illumination. Pairs of flashes were given with a short, preset time interval between both flashes, yielding in one TV picture two images of the same cell displaced over a certain distance for the given time interval. Velocity and mean radial position of cells, flowing within an optical section around the median plane of the vessel, were determined. The shape of the velocity profiles of platelets and red blood cells was similar. The profiles were flattened as compared to a parabola, both in syustole an diastole. Vessel diameter did not change measurably during the cardiac cycle. As an index of the degree of blunting of the profiles, the ratio of the maximal and mean velocity of the profile was used, which is 2 for a parabola and 1 for complete plug flow. The index ranged from1.39 to 1.54 (median 1.50), and increased with vessel diameter. Calculations showed that the blunting of the profiles cannot be explained by an influence of the finite depth of the optical section.