Glial cells metabolically cooperate: a potential requirement for gene replacement therapy.
Glial cells metabolically cooperate: a potential requirement for gene replacement therapy.
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神经胶质细胞代谢协作：基因替代疗法的潜在要求。
DOI：
10.1073/pnas.82.19.6662
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发表时间：
1985
影响因子：
11.1
通讯作者：
Seegmiller,JE
中科院分区：
文献类型：
--
作者：
Gruber,HE;Koenker,R;Luchtman,LA;Willis,RC;Seegmiller,JE
放射自显像显示免疫荧光标记的胶质细胞在代谢上与次黄嘌呤磷酸核糖转移酶缺陷的成纤维细胞合作。在没有细胞接触的情况下观察到的合作和在胶质细胞周围的结合梯度表明，合作是通过放射性标记嘌呤化合物的细胞外运输发生的。当受体细胞也缺乏腺嘌呤或腺苷回收所需的酶时，放射性标记腺嘌呤、腺苷或甲基硫代腺苷的转移是由合作的定量损失支持的。胶质细胞合作的证明为目前对Lesch-Nyhan综合征神经系统症状的基因替代疗法的研究提供了动力。
Immunofluorescently labeled glial cells are shown by radioautography to metabolically cooperate with hypoxanthine phosphoribosyltransferase-deficient fibroblasts. The observations of cooperation without cell contact and of incorporation gradients around the glial cells suggest that cooperation occurs through extracellular transport of radiolabeled purine compounds. The transfer of radiolabeled adenine, adenosine, or methylthioadeninosine is supported by the quantitative loss of cooperation when the recipient cell is also deficient in enzymes required for adenine or adenosine salvage. The demonstration of glial cell cooperation provides impetus for current research toward gene replacement therapy for the neurologic symptoms of the Lesch-Nyhan syndrome.