MOLECULAR ASSOCIATION BETWEEN TRANSPLANTATION ANTIGENS AND CELL-SURFACE ANTIGEN IN ADENOVIRUS-TRANSFORMED CELL LINE
MOLECULAR ASSOCIATION BETWEEN TRANSPLANTATION ANTIGENS AND CELL-SURFACE ANTIGEN IN ADENOVIRUS-TRANSFORMED CELL LINE
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DOI：
10.1073/pnas.75.11.5674
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
PETERSON, PA
中科院分区：
文献类型：
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作者：
KVIST, S;OSTBERG, L;PETERSON, PA
用2型腺病毒转化的大鼠细胞系（A2 T2 C4）在同基因大鼠中引起细胞毒性T [胸腺衍生]淋巴细胞。针对主要组织相容性（AgB）抗原的兔抗血清和针对病毒转化细胞系的同系大鼠抗血清消除了细胞毒性。同源抗血清免疫沉淀表面蛋白，表观分子量分别为45，000、19，000、17，000和12，000，来自A2 T2 C4细胞，但对原代大鼠成纤维细胞和脾细胞无反应性。抗AgB抗原的兔抗血清从A2 T2 C4细胞中沉淀出19，000-道尔顿的组分，而在原代大鼠成纤维细胞中未观察到。顺序免疫沉淀显示识别的2个抗血清的主要多肽之间的身份。因为兔抗AgB抗原血清对移植抗原亚单位是特异性的，并且因为抗A2 T2 C4细胞的同系大鼠抗血清不能与正常细胞中的AgB抗原反应，所以19，000-道尔顿组分显然与AgB抗原共沉淀。特异性针对β 2-微球蛋白和同种抗原性AgB抗原亚单位的抗血清也共沉淀19，000-道尔顿组分。AgB抗原亚单位显然与病毒转化的A2 T2 C4细胞表面上的病毒编码蛋白形成三元复合物。这种分子复合物可被溶细胞性T淋巴细胞识别。
A rat cell line (A2T2C4), transformed with adenovirus type 2, elicited cytotoxic T [thymus derived] lymphocytes in syngeneic rats. Cytotoxicity was abolished by a rabbit antiserum directed against the major histocompatibility (AgB) antigens and by a syngeneic rat antiserum raised against the virus-transformed cell line. The syngeneic antiserum immunoprecipitated surface proteins with apparent MW of 45,000, 19,000, 17,000 and 12,000 from the A2T2C4 cells but it displayed no reactivity against primary rat fibroblasts and spleen cells. The rabbit antiserum against AgB antigens precipitated a 19,000-dalton component from the A2T2C4 cells, which was not observed in primary rat fibroblasts. Sequential immunoprecipitation revealed identity between the major polypeptides recognized by the 2 antisera. Because the rabbit anti-AgB antigen serum was specific for the transplantation antigen subunits and because the syngeneic rat antiserum against the A2T2C4 cells failed to react with the AgB antigens in normal cells, the 19,000-dalton component is apparently coprecipitated with the AgB antigens. Antisera directed specifically against .beta.2-microglobulin and the alloantigenic AgB antigen subunit also coprecipitated the 19,000-dalton component. The AgB antigen subunits apparently form a ternary complex with a virus-coded protein on the surface of the virus-transformed A2T2C4 cells. This molecular complex may be recognized by the cytolytic T lymphocytes.