Postpartum-activation of multiple sclerosis is associated with down-regulation of tolerogenic HLA-G
Postpartum-activation of multiple sclerosis is associated with down-regulation of tolerogenic HLA-G
复制标题
DOI：
10.1016/j.jneuroim.2007.05.008
复制
发表时间：
2007-07-01
影响因子：
3.3
通讯作者：
Wiendl, Heinz
中科院分区：
文献类型：
--
作者：
Airas, Laura;Nikula, Tuomas;Wiendl, Heinz
我们使用微阵列分析来深入了解控制妊娠相关多发性硬化症活动的免疫调节机制。我们研究了妊娠期和产后复发缓解期MS患者外周血单核细胞中5000个免疫相关基因的表达水平，并与对照组进行了比较。在基因芯片分析中，发现主要具有免疫耐受功能的非经典主要组织相容性分子--人类白细胞抗原-G在MS患者和对照组之间存在差异调节。这一发现得到了实时聚合酶链式反应、流式细胞仪和酶联免疫吸附试验在更大的患者样本中的证实和扩展。这一结果揭示了免疫耐受分子HLA-G在调节MS患者的疾病活动和妊娠相关变化中的重要作用。(C)2007 Elsevier B.V.保留所有权利。
We used microarray analysis to obtain insights into the immuno-regulatory mechanisms controlling pregnancy-associated MS disease activity. We studied expression levels of 5000 immune-related genes in peripheral blood mononuclear cells in patients with relapsing-remitting MS during pregnancy and postpartum and in comparison to controls. In the microarray analysis, HLA-G, a non-classical major histocornpatibility molecule mainly attributed with immune-tolerogenic functions, was found differentially regulated between MS patients and controls. The finding was corroborated and extended by real-time PCR, flow-cytometry and ELISA in a larger patient sample. The results delineate an important role for the immune-tolerogenic molecule HLA-G in modulating disease activity and pregnancy-related changes in MS patients. (c) 2007 Elsevier B.V. All rights reserved.