The impact of maceration on the 'Osteo-ome'; a pilot investigation.
The impact of maceration on the 'Osteo-ome'; a pilot investigation.
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浸渍对“Osteo-ome”的影响；
DOI：
10.1016/j.jprot.2022.104754
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发表时间：
2023
影响因子：
3.3
通讯作者：
Gent L
中科院分区：
文献类型：
--
作者：
Gent L
骨蛋白质组，即，是法医研究的丰富信息来源。通过在肽水平上观察蛋白质丰度和翻译后修饰（PTM）的变化，在用于死亡年龄（AAD）和死后间隔（PMI）估计的生物分子生物标志物的研究中已经取得了进展。然而，其他死后因素改变蛋白质组的程度，包括在人类埋藏设施（HTFs）中采用的“浸渍”程序，以清洁骨骼，用于骨骼学收集，知之甚少。这项初步研究旨在确定这些“清洁”方法对骨骼生物分子的影响，以及在未来的研究中这可能对推定的生物标志物产生的进一步影响。对来自三只牛的六只牛的骨骼进行了三种特定的浸泡程序，浸泡时间（一周或两天）和水温（55 °C或87 °C）各不相同;比较了每只牛的新鲜骨和浸泡骨的蛋白质组。在87 °C下浸渍两天具有最大的蛋白质组学影响，降低蛋白质相对丰度并诱导特异性PTM。总体而言，这些结果表明，受雇于浸渍程序是苛刻的，变量和潜在的威胁的可行性，发现新的法医生物标志物在浸渍的骨骼remain.SignificanceFor第一次，骨蛋白质组学的应用在理解浸渍程序进行，以帮助解决与这种死后清洗技术相关的实验混淆的风险。这项初步研究表明，最近的进展，生物标志物发现的死后间隔和死亡年龄估计使用骨蛋白质组学有可能混淆不同的和破坏性的骨清洁方法。
The bone proteome,i.e.,the ‘osteo-ome’, is a rich source of information for forensic studies. There have been advances in the study of biomolecule biomarkers for age-at-death (AAD) and post-mortem interval (PMI) estimations, by looking at changes in protein abundance and post-translational modifications (PTMs) at the peptide level. However, the extent to which other post-mortem factors alter the proteome, including ‘maceration’ procedures adopted in human taphonomy facilities (HTFs) to clean bones for osteological collections, is poorly understood. This pilot study aimed to characterise the impact of these ‘cleaning’ methods for de-fleshing skeletons on bone biomolecules, and therefore, what further impact this may have on putative biomarkers in future investigations. Three specific maceration procedures, varying in submersion time (one week or two days) and water temperature (55 °C or 87 °C) were conducted on six bovid tibiae from three individual bovines; the proteome of fresh and macerated bones of each individual was compared. The maceration at 87 °C for two days had the greatest proteomic impact, decreasing protein relative abundances and inducing specific PTMs. Overall, these results suggest that routinely-employed maceration procedures are harsh, variable and potentially threaten the viability of discovering new forensic biomarkers in macerated skeletal remains.SignificanceFor the first time, the application of bone proteomics in understanding maceration procedures was conducted to help address the risks for experimental confounding associated with this post-mortem cleaning technique. This pilot study demonstrates that recent advances in biomarker discovery for post-mortem interval and age-at-death estimation using bone proteomics has potential for confounding by differing and destructive bone-cleaning methods.