ICAM-1 targeting of doxorubicin-loaded PLGA nanoparticles to lung epithelial cells.
ICAM-1 targeting of doxorubicin-loaded PLGA nanoparticles to lung epithelial cells.
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DOI：
10.1016/j.ejps.2009.02.008
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发表时间：
2009-05-12
影响因子：
4.6
通讯作者：
Berkland, Cory J.
中科院分区：
文献类型：
--
作者：
Chittasupho, Chuda;Xie, Sheng-Xue;Baoum, Abdulgader;Yakovleva, Tatyana;Siahaan, Teruna J.;Berkland, Cory J.
T淋巴细胞上的白细胞功能相关抗原-1(LFA-1)和上皮细胞上的细胞间黏附分子-1(ICAM-1)相互作用，控制着白细胞的黏附、扩散和渗出。这一过程在白细胞重新聚集到特定的炎症部位起着重要作用，并已被确认为某些类型癌症的生物标志物。环-(1，12)-PenITDGEATDSGC(CLABL)通过与ICAM-1结合来抑制LFA-1和ICAM-1的相互作用。此外，cLABL已被证明在与ICAM-1结合后内化。本研究探讨了cLABL共轭纳米粒(cLABL-NP)作为靶向控释给药系统的可能性。在聚乳酸-乙醇酸共聚纳米粒(PLGA)表面修饰Pluronic®表面活性剂后，将cLABL多肽偶联到PLGA上。与不含多肽的纳米粒相比，cLABL-NP对A549肺上皮细胞的摄取速度更快。用游离的cLABL多肽阻断细胞表面cLABL-NP与ICAM-1的结合，通过阻断cLABL-NP对ICAM-1的摄取，证实了ICAM-1介导的内化的特异性。细胞研究表明，cLABL-NPs靶向包埋阿霉素的ICAM-1表达细胞。细胞毒性实验证实了纳米粒中药物的活性。由PLGA纳米粒提供的阿霉素的持续释放可能使cLABL-NP成为靶向、控制释放的药物输送系统。
Interaction of leukocyte function associated antigen-1 (LFA-1) on T-lymphoctytes and intercellular adhesion molecule-1 (ICAM-1) on epithelial cells controls leukocyte adhesion, spreading, and extravasation. This process plays an important role in leukocyte recruitment to a specific site of inflammation and has been indentified as a biomarker for certain types of carcinomas. Cyclo-(1,12)-PenITDGEATDSGC (cLABL) has been shown to inhibit LFA-1 and ICAM-1 interaction via binding to ICAM-1. In addition, cLABL has been shown to internalize after binding ICAM-1. The possibility of using cLABL conjugated nanoparticles (cLABL-NP) as a targeted and controlled release drug delivery system has been investigated in this study. The cLABL peptide was conjugated to a modified Pluronic® surfactant on poly (DL-lactic-co-glycolic acid) (PLGA) nanoparticles. The cLABL-NP showed more rapid cellular uptake by A549 lung epithelial cells compared to nanoparticles without peptide. The specificity of ICAM-1 mediated internalization was confirmed by blocking the uptake of cLABL-NP to ICAM-1 using free cLABL peptide to block the binding of cLABL-NP to ICAM-1 on the cell surface. Cell studies suggested that cLABL-NPs targeted encapsulated doxorubicin to ICAM-1 expressing cells. Cytotoxicity assay confirmed the activity of the drug incorporated in nanoparticles. Sustained release of doxorubicin afforded by PLGA nanoparticles may enable cLABL-NP as a targeted, controlled release drug delivery system.
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影响因子：
4.9
作者：
Duvvuri, Muralikrishna;Krise, Jeffrey P.
通讯作者：
Krise, Jeffrey P.
影响因子：
10.8
作者：
Kataoka, K;Matsumoto, T;Kwon, GS
通讯作者：
Kwon, GS
影响因子：
29.4
作者：
Kim, Jaeyun;Lee, Ji Eun;Hyeon, Taeghwan
通讯作者：
Hyeon, Taeghwan
DOI：
10.1073/pnas.0712202105
发表时间：
2008-05-20
影响因子：
11.1
作者：
Kurosawa, Gene;Akahori, Yasushi;Kurosawa, Yoshikazu
通讯作者：
Kurosawa, Yoshikazu
影响因子：
14
作者：
Cheng, Jianjun;Teply, Benjamin A.;Farokhzad, Omid C.
通讯作者：
Farokhzad, Omid C.