Experimental bovine coronavirus in turkey poults and young chickens
Experimental bovine coronavirus in turkey poults and young chickens
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DOI：
10.2307/1593023
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发表时间：
2001-01-01
期刊：
影响因子：
1.4
通讯作者：
Saif, YM
中科院分区：
文献类型：
--
作者：
Ismail, MM;Cho, KO;Saif, YM
在三个试验中，使用牛冠状病毒（BCV）的DB2小牛株来感染1日龄无特定病原体（SPF）火鸡幼雏。在所有试验中，鸡在接种后48-72小时出现肠炎临床体征。在接种后3、5和7天（DPI）处死的鸡具有松弛、苍白的肠道，内容物为水样，盲肠明显增大，内容物为泡沫。冠状病毒颗粒通过免疫电子显微镜检测与BCV抗体从禽肠道内容物在3，5，7，和12 DPI杀死。与对照组相比，在3、5和7 DPI处死的接种家禽的体重显著降低。在12 DPI的恢复期鸟类血清中检测到血凝抗体。然而，在两项试验中，用相同的病毒对1日龄SPF鸡进行实验性接种，未导致临床体征或肉眼或显微镜病变。从肠道内容物中未检测到冠状病毒，接种和未接种对照鸡的体重无显著差异。
The DB2 calf strain of bovine coronavirus (BCV) was used to inoculate 1-day-old specific-pathogen-free (SPF) turkey poults in three trials. In all trials, the birds developed clinical signs of enteritis at 48-72 hr postinoculation. Birds euthanatized at 3, 5, and 7 days postinoculation (DPI) had flaccid, pale intestines with watery contents, and the ceca were markedly enlarged with frothy contents. Coronavirus particles were detected by immune electron microscopy with BCV antibodies from the intestinal contents of birds killed at 3, 5, 7, and 12 DPI. Body weights of inoculated poults killed at 3, 5, and 7 DPI were significantly reduced as compared with controls. Hemagglutinating antibodies were detected in sera of convalescent birds at 12 DPI. However, experimental inoculation of 1-day-old SPF chicks in two trials with the same virus resulted in no clinical signs or macroscopic or microscopic lesions. No coronaviruses were detected from intestinal contents, and there were no significant differences in body weights of inoculated and noninoculated control chicks.