A SIMPLE SERUM-FREE FREEZING MEDIUM FOR SERUM-FREE CULTURED-CELLS
A SIMPLE SERUM-FREE FREEZING MEDIUM FOR SERUM-FREE CULTURED-CELLS
复制标题
DOI：
10.1006/biol.1995.0030
复制
发表时间：
1995-06-01
期刊：
影响因子：
1.7
通讯作者：
COUVE, E
中科院分区：
文献类型：
--
作者：
MERTEN, OW;PETRES, S;COUVE, E
由于细胞培养物中血清的存在会给生物制品的生产带来安全问题，因此我们开发了一种用于动物细胞冷冻保存的无血清培养基 (SFM)。该培养基基于SFM MDSS2，其中添加10%二甲基亚砜（DMSO）和0.1%甲基纤维素或3%聚乙烯吡咯烷酮或除DMSO外不添加其他添加剂。在 MDSS2 中定期培养的 Vero 和 BHK-21 细胞都可以在三种无血清冷冻介质中冷冻保存，并且可以解冻而不会造成任何细胞损失。标准冷冻培养基（含有 10% 胎牛血清和 10% DMSO 的 DMEM）中的细胞与 SFM 中冷冻的细胞在细胞生长和活力方面没有发现差异。就 Vero 细胞而言，在其附着方面没有观察到差异。该培养基代表了完成基于无血清动物细胞的生物过程的最后一步。
Because the presence of serum in cell culture raises safety problems for the production of biologicals, we have developed a serum-free medium (SFM) for the cryopreservation of animal cells. This medium is based on the SFM MDSS2, to which 10% dimethylsulfoxide (DMSO) and 0.1% methylcellulose or 3% polyvinyl pyrrolidone or no other additive than DMSO were added. Both, Vero and BHK-21 cells regularly cultivated in MDSS2, could be cryopreserved in the three serum-free freezing media and could be thawed without any cell loss. No differences could be found between the cells in the standard freezing medium (DMEM containing 10% fetal calf serum and 10% DMSO) and those frozen in SFM, with respect to cell growth and viability. In the case of the Vero cells no differences were observed with respect to their attachment. This medium represents the last step in fulfilling a complete serum-free animal cell based bioprocess.