Stable immobilization of an oligonucleotide probe on a gold substrate using tripodal thiol derivatives
Stable immobilization of an oligonucleotide probe on a gold substrate using tripodal thiol derivatives
复制标题
DOI：
10.1021/la0616193
复制
发表时间：
2007-02-27
期刊：
影响因子：
3.9
通讯作者：
Miyahara, Yuji
中科院分区：
文献类型：
--
作者：
Sakata, Toshiya;Maruyama, Sumio;Miyahara, Yuji
我们提出了一种将DNA探针固定在DNA芯片固体底物上的界面分子。我们设计并合成了三脚架硫醇衍生物，用于稳定地将寡核苷酸探针固定在金表面。基于三脚架的四面体结构，将三脚架上的硫醇衍生物垂直连接到金衬底上，从而控制寡核苷酸探针的方向。当带有硫醇衍生物连接的寡核苷酸探针的金衬底暴露在更高温度的去离子水中时，三脚架界面分子比单一接触分子更稳定地附着在金表面。该DNA芯片平台与功能界面分子相结合，适合于临床诊断中用于基因分析的可重复性、廉价、高通量的检测系统。
We proposed an interface molecule for immobilization of DNA probes on solid substrates of DNA chips. We have designed and synthesized tripodal thiol derivatives for stable immobilization of oligonucleotide probes on a gold surface. On the basis of the tetrahedral structure of tripod, the tripodal thiol derivatives were bonded upright to the gold substrate, which would control the orientation of oligonucleotide probes. When the gold substrate with oligonucleotide probes tethered using the thiol derivatives was exposed to deionized water at higher temperatures, the tripodal interface molecules were attached to the gold surface more stably than the single contact molecules. The DNA chip platform combined with the functional interface molecule is suitable for a reproducible, inexpensive, and high-throughput detection system for genetic analyses in clinical diagnostics.