Dimensionality of diffusive exploration at the protein interface in solution.
Dimensionality of diffusive exploration at the protein interface in solution.
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DOI：
10.1021/jp9048082
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发表时间：
2009-10-08
期刊：
影响因子：
--
通讯作者：
Bryant RG
中科院分区：
文献类型：
--
作者：
Grebenkov DS;Goddard YA;Diakova G;Korb JP;Bryant RG
水的动力学对蛋白质和底物之间的相互作用能量至关重要，并决定了界面上的传输效率。核自旋-晶格驰豫速率常数1/t1与磁场的依赖关系提供了水在蛋白质界面扩散动力学的直接表征。我们发现，表面平均平移关联时间为30-40ps，水质子1/T1的磁场依赖关系是水在蛋白质界面区的二维扩散。降维大大增加了分子间重遇的概率和小分子表面探索的效率，在这种情况下是水。我们提出了一个关于球形大分子附近小扩散粒子平移效应的综合理论，它是两个粒子相对大小的函数。我们表明，扩散探测的视维的变化是小扩散粒子与大得多的粒子相遇的一般结果，大得多的粒子呈现扩散障碍。对限制的大小相对于大分子大小的影响的检验表明，表征小分子扩散的降维保持在非常小的半径比。对几种不同蛋白质在溶液中的实验结果支持所提出的理论模型，该模型可推广到其他小颗粒-大体系统，如囊泡和胶束。
Dynamics of water are critically important to the energies of interaction between proteins and substrates and determine the efficiency of transport at the interface. The magnetic field dependence of nuclear spin-lattice relaxation rate constant 1/T1 of water protons provides a direct characterization of water diffusional dynamics at the protein interface. We find that the surface-average translational correlation time is 30–40 ps, and the magnetic field dependence of the water-proton 1/T1 is characteristic of 2-dimensional diffusion of water in the protein interfacial region. The reduced dimensionality substantially increases the intermolecular reencounter probability and the efficiency of the surface exploration by the small molecule, water in this case. We propose a comprehensive theory of the translational effects of a small diffusing particle confined in the vicinity of a spherical macromolecule as a function of the relative size of the two particles. We show that the change in the apparent dimensionality of the diffusive exploration is a general result of the small diffusing particle encountering a much larger particle that presents a diffusion barrier. Examination of the effects of the size of the confinement relative to the macromolecule size reveals that the reduced dimensionality characterizing the small molecule diffusion persists to remarkably small radius ratios. The experimental results on several different proteins in solution support the proposed theoretical model that may be generalized to other small particle-large body systems like vesicles and micelles.
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影响因子：
2.3
作者：
Dellerue, S;Bellissent-Funel, MC
通讯作者：
Bellissent-Funel, MC
影响因子：
3.3
作者：
Bandyopadhyay, S;Chakraborty, S;Bagchi, B
通讯作者：
Bagchi, B
影响因子：
2.4
作者：
Godefroy, S;Korb, JP;Bryant, RG
通讯作者：
Bryant, RG
DOI：
10.1209/epl/i1997-00128-3
发表时间：
1997-01-20
期刊：
EUROPHYSICS LETTERS
影响因子：
--
作者：
Bizzarri, AR;Cannistraro, S
通讯作者：
Cannistraro, S
影响因子：
2.9
作者：
HALLENGA, K;KOENIG, SH
通讯作者：
KOENIG, SH