Rinderpest virus H protein: role in determining host range in rabbits.
Rinderpest virus H protein: role in determining host range in rabbits.
复制标题
牛瘟病毒 H 蛋白：在确定兔子宿主范围中的作用。
作者：
M. Yoneda;S. Bandyopadhyay;M. Shiotani;K. Fujita;A. Nuntaprasert;R. Miura;M. Baron;T. Barrett;C. Kai
病毒宿主范围的一个主要分子决定因素被认为是细胞附着所需的病毒蛋白。我们使用Rinderest病毒(RPV)的重组株来检测该蛋白在确定RPV在兔体内复制能力方面的作用。重组疫苗是基于兔无毒力的RBOK疫苗株，携带兔狂犬病疫苗株(RPV-L株)的血凝素(H)蛋白基因。重组病毒(rRPV-LAPH)是从RPV-L株的H基因片段中回收的重组病毒。重组病毒在B95a细胞中的生长速度与RPV-RBOK亲本病毒相当，但低于RPV-L。H基因互换不影响RBOK病毒作为疫苗保护牛抵御RPV强毒攻击的能力。兔接种狂犬病病毒L后出现发热，体重增加减少，白细胞减少。在淋巴组织中也观察到高病毒滴度和组织病理学损害。接种RPV-RBOK或rRPV-LAPH的兔均未观察到感染的临床症状，但与RPV-RBOK不同的是，RPV-L和rRPV-LAPH均能诱导兔产生明显的抗体应答。因此，H蛋白在允许兔感染方面发挥了重要作用，但显然需要其他病毒蛋白才能在该物种中表现出完全的RPV致病性。
A major molecular determinant of virus host-range is thought to be the viral protein required for cell attachment. We used a recombinant strain of Rinderpest virus (RPV) to examine the role of this protein in determining the ability of RPV to replicate in rabbits. The recombinant was based on the RBOK vaccine strain, which is avirulent in rabbits, carrying the haemagglutinin (H) protein gene from the lapinized RPV (RPV-L) strain, which is pathogenic in rabbits. The recombinant virus (rRPV-lapH) was rescued from a cDNA of the RBOK strain in which the H gene was replaced with that from the RPV-L strain. The recombinant grew at a rate equivalent to the RPV-RBOK parental virus in B95a cells but at a lower rate than RPV-L. The H gene swap did not affect the ability of the RBOK virus to act as a vaccine to protect cattle against virulent RPV challenge. Rabbits inoculated with RPV-L became feverish, showed a decrease in body weight gain and leukopenia. High virus titres and histopathological lesions in the lymphoid tissues were also observed. Clinical signs of infection were never observed in rabbits inoculated with either RPV-RBOK or with rRPV-lapH; however, unlike RPV-RBOK, both RPV-L and rRPV-lapH induced a marked antibody response in rabbits. Therefore, the H protein plays an important role in allowing infection to occur in rabbits but other viral proteins are clearly required for full RPV pathogenicity to be manifest in this species.