Sinus culture poorly predicts resident microbiota
Sinus culture poorly predicts resident microbiota
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DOI：
10.1002/alr.21428
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发表时间：
2015-01-01
影响因子：
6.4
通讯作者：
Ramakrishnan, Vijay R.
中科院分区：
文献类型：
--
作者：
Hauser, Leah J.;Feazel, Leah M.;Ramakrishnan, Vijay R.
BackgroundChronic rhinosinusitis (CRS) is an inflammatory disorder of the paranasal sinuses in which bacteria are implicated. Culture-based assays are commonly used in clinical and research practice; however, culture conditions may not accurately detect the full range of microorganisms present in a sample. The objective of this study was to determine the accuracy of clinical culture of CRS specimens compared with DNA-based molecular techniques.MethodsEthmoid samples from 54 CRS patients collected during endoscopic sinus surgery were analyzed by both clinical culture and 16S ribosomal RNA (rRNA) gene sequencing. The association between 16S relative abundance and detection by culture was determined using logistic regression.ResultsEach subject had an average of 3 isolates identified by bacterial culture and 21.5 12.5 species identified by 16S sequencing. On average, 1.6 dominant taxa (>10% abundance) per subject were identified using molecular techniques, but only 47.7% of these taxa were identified by culture. Low abundance taxa (abundance
