iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum.
iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum.
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DOI：
10.1093/nar/gku1054
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发表时间：
2015-01
影响因子：
14.9
通讯作者：
Bucher G
中科院分区：
文献类型：
--
作者：
Dönitz J;Schmitt-Engel C;Grossmann D;Gerischer L;Tech M;Schoppmeier M;Klingler M;Bucher G
iBeetle-Base（http：ibeetle-base.uni-goettingen.de）提供RNAi表型的注释，其在红粉甲虫赤拟谷盗（Tribolium castaneum）中的大规模RNAi筛选（iBeetle筛选）中收集。此外，它还提供了所有赤拟谷盗基因的序列信息和链接。iBeetle-Base包含在胚胎和变态表皮和肌肉发育期间敲除的数千个基因的表型注释，以及与卵子发生和臭腺生物学相关的表型。根据EQM（实体，质量，修饰符）系统，使用受控词汇表和Tribolium形态本体（Tron）的表型进行描述。此外，提供链接到相应注释的图像。这些数据可以通过复杂的“形态缺陷搜索”或“快速搜索”基因名称和ID来搜索特定的表型。赤拟谷盗Tribolium castaneum已成为昆虫功能遗传学研究的重要模式系统，是鞘翅目昆虫中物种最丰富的类群之一。用于研究昆虫典型发育、发育进化以及代谢和害虫防治研究。除果蝇外，赤拟谷盗是第一个进行大规模无偏筛选的昆虫模式生物。
The iBeetle-Base (http://ibeetle-base.uni-goettingen.de) makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (iBeetle screen). In addition, it provides access to sequence information and links for all Tribolium castaneum genes. The iBeetle-Base contains the annotations of phenotypes of several thousands of genes knocked down during embryonic and metamorphic epidermis and muscle development in addition to phenotypes linked to oogenesis and stink gland biology. The phenotypes are described according to the EQM (entity, quality, modifier) system using controlled vocabularies and the Tribolium morphological ontology (TrOn). Furthermore, images linked to the respective annotations are provided. The data are searchable either for specific phenotypes using a complex ‘search for morphological defects’ or a ‘quick search’ for gene names and IDs. The red flour beetle Tribolium castaneum has become an important model system for insect functional genetics and is a representative of the most species rich taxon, the Coleoptera, which comprise several devastating pests. It is used for studying insect typical development, the evolution of development and for research on metabolism and pest control. Besides Drosophila, Tribolium is the first insect model organism where large scale unbiased screens have been performed.
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影响因子：
4.5
作者：
Li J;Lehmann S;Weißbecker B;Ojeda Naharros I;Schütz S;Joop G;Wimmer EA
通讯作者：
Wimmer EA
影响因子：
14.9
作者：
Grumbling G;Strelets V
通讯作者：
Strelets V
本体论的实际应用在注释和分析大规模的原始小鼠表型数据。
影响因子：
3
作者：
Beck T;Morgan H;Blake A;Wells S;Hancock JM;Mallon AM
通讯作者：
Mallon AM
影响因子：
4.5
作者：
Posnien N;Koniszewski ND;Hein HJ;Bucher G
通讯作者：
Bucher G
DOI：
10.1073/pnas.1116641109
发表时间：
2012-05-15
影响因子：
11.1
作者：
Fu, Jinping;Posnien, Nico;Bucher, Gregor
通讯作者：
Bucher, Gregor