Human c-Ki-ras2 proto-oncogene on chromosome 12.
Human c-Ki-ras2 proto-oncogene on chromosome 12.
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人类 c-Ki-ras2 原癌基因位于 12 号染色体上。
DOI：
10.1126/science.6823569
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Weinberg,RA
中科院分区：
文献类型：
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作者：
Sakaguchi,AY;Naylor,SL;Shows,TB;Toole,JJ;McCoy,M;Weinberg,RA
最近被鉴定为Kirsten肉瘤基因（v-ras）的细胞同源物的人结肠腺癌转化基因被用于通过Southern杂交方法将人细胞Kirstenras 2基因分配到12号染色体。通过DNA转染和分子克隆分离的转化基因的单个640个碱基对的Eco RI-Hind III片段可以检测来自表型正常细胞的DNA的单个Eco RI片段（2.9个内切酶对）。这些数据表明c-Ki-ras 2的恒定染色体位置。
A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (v-ras), was used to assign the human cellular Kirstenras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI—Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.