Extracellular recordings of bionic engineered cardiac tissue based on a porous scaffold and microelectrode arrays
Extracellular recordings of bionic engineered cardiac tissue based on a porous scaffold and microelectrode arrays
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基于多孔支架和微电极阵列的仿生工程心脏组织的细胞外记录
DOI：
10.1039/c9ay01888c
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发表时间：
2019
影响因子：
3.1
通讯作者：
Ping Wang
中科院分区：
文献类型：
--
作者：
Xinwei Wei;Qing Gao;Chaoqi Xie;Chenlei Gu;Tao Liang;Hao Wan;Liujing Zhuang;Yong He;Ping Wang
心血管疾病仍然是威胁人类生命和健康的头号疾病，但目前还缺乏一种强有力的体外模拟心脏的方法来研究心血管疾病的药物治疗机制。本工作构建了基于多孔支架的仿生工程心脏组织(ECT)，并利用微电极阵列(MEAs)进行细胞外电生理记录。以聚乳酸和聚己内酯为载体，采用3D打印和静电纺丝的方法制备多孔支架，然后将新生SD大鼠心肌细胞培养在聚乳酸/己内酯支架上，构建电休克断层扫描支架。通过细胞活性和机械敲击状态的检测，验证了支架的强度和生物相容性。设计了一套MEA传感器和自制的检测系统，并将ECT与MEA传感器耦合进行电生理评价。通过注射异丙肾上腺素(ISO)来验证ECT的功能。这项工作为ECT的评价提供了一种新的方法，有望应用于药学研究。
Cardiovascular disease remains the leading threat to human life and health, however, a powerful method to mimic the heart in vitro to study the mechanisms of drug therapy on cardiovascular disease is still lacking. In this work, bionic engineered cardiac tissue (ECT) based on a porous scaffold was fabricated, and microelectrode arrays (MEAs) were utilized for extracellular electrophysiological recording. Polylactic acid (PLA) and poly-ε-caprolactone (PCL) were used to fabricate the porous scaffold via 3D printing and electrospinning, and then cardiomyocytes from neonatal Sprague Dawley (SD) rats were cultured on the PLA/PCL scaffold to construct the ECT. The strength and biocompatibility of the scaffold were verified via evaluating the cell viability and mechanical beating status. A set of MEA sensors and a home-made detection system were designed, and the ECT was coupled with the MEA sensors for electrophysiological evaluation. The functionality of the ECT was verified via administrating isoprenaline (ISO). This work provides a new approach for evaluating ECT, which is expected to be applied to pharmaceutical studies.