Real-time PCR quantification of latent infection of wheat powdery mildew in the field
Real-time PCR quantification of latent infection of wheat powdery mildew in the field
复制标题
田间小麦白粉病潜伏感染的实时PCR定量
DOI：
10.1007/s10658-013-0188-5
复制
发表时间：
2013-07-01
影响因子：
1.8
通讯作者：
Wang, Baotong
中科院分区：
文献类型：
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作者：
Zheng, Yaming;Luo, Yong;Wang, Baotong
小麦白粉病，由真菌病原菌blumeria graminissp引起。小麦黑穗病（Bgt）是一种世界性的破坏性小麦病害。病害预报的关键问题是及时准确地估计和量化志愿苗木中病原越冬或越夏的潜伏感染水平，作为疫病的初始接种源。为了改进传统方法，本研究建立了小麦叶片潜伏侵染水平的实时荧光定量PCR方法。收集了中国不同地理位置的人工和自然侵染的小麦叶片，并对其进行处理，以确定潜伏侵染水平。通过人工接种实验，得到了分子病害指数（MDX）与观测病害指数（DX）之间的线性关系。田间试验结果表明，MDX与DX的空间分布格局在大多数情况下吻合较好。本研究表明，实时荧光定量PCR是快速、准确地定量小麦白粉病潜伏感染水平和有效估计田间流行初始接种势的有效工具。
Wheat powdery mildew, caused by the fungal pathogenBlumeria graminisf.sp. tritici(Bgt), is a destructive wheat disease worldwide. The key issue for the disease forecast is to timely and accurately estimate and quantify the latent infection levels in volunteer seedlings where the pathogen over-winters or over-summers to serve as sources of initial inoculum of epidemics. To improve the conventional method, a real-time PCR assay had been established in this study to quantify latent infection level of wheat leaves. Artificially and naturally infected leaves in wheat fields at different geographical locations in China were collected and processed to determine the latent infection levels. Linear relationships between the molecular disease index (MDX) and the observed disease index (DX) were obtained from artificial inoculation experiments. Field experiments showed that the spatial distribution patterns of MDX matched well with those of DX in the most cases. This study demonstrated that the real-time PCR assay was a useful tool to rapidly and accurately quantify the latent infection levels of wheat powdery mildew and to efficiently estimate the initial inoculum potentials of epidemics in the fields.