Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target
Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target
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DOI：
10.1016/j.bbrc.2017.07.161
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发表时间：
2017-09-30
影响因子：
3.1
通讯作者：
Tokumitsu, Hiroshi
中科院分区：
文献类型：
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作者：
Sakane, Kyohei;Nishiguchi, Miyu;Tokumitsu, Hiroshi
S100A6是一种Ca2+信号传感器，可与多种蛋白质相互作用并调节其生化功能。本研究通过筛选携带19,676个重组gst融合人蛋白的蛋白微阵列，鉴定了一个中心体蛋白FOR20 （20 kDa的fop相关蛋白）作为新的S100A6靶点。结合实验表明，S100A6在体外和活细胞中以Ca2+依赖的方式与FOR20的n端区（残基1-30）相互作用。包括S100A1、A2、A4、All、B在内的几个S100蛋白也与FOR20和S100A6表现出Ca2+依赖性相互作用。我们发现两个远亲的中心体蛋白，FOP和OFD1，也具有与FOR20中假定的S100A6结合位点（残基1-30）具有显著序列相似性的n端区域，并且能够以Ca2+依赖的方式与S100A6结合。综上所述，这些结果可能表明S100A6通过一个保守的n端结构域与FOR20和相关的中心体蛋白相互作用，提示一种新的Ca2+依赖性中心体功能调控。(C) 2017爱思唯尔公司版权所有。
S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal region (residues 1-30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, All, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1-30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function. (C) 2017 Elsevier Inc. All rights reserved.