Molecular cloning and sequence analysis of trp-lac fusion deletions.
Molecular cloning and sequence analysis of trp-lac fusion deletions.
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trp-lac 融合缺失的分子克隆和序列分析。
DOI：
10.1016/s0022-2836(84)80032-7
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发表时间：
1984
影响因子：
5.6
通讯作者：
Reznikoff,WS
中科院分区：
文献类型：
--
作者：
Yu,XM;Munson,LM;Reznikoff,WS
克隆了含有将troperon融合到thelacoperon的缺失的DNA片段，并确定了融合的终点。DNA序列分析的结果与以前报道的遗传、生化和生理学研究的结果相关性很好。本研究的序列数据，结合这些融合菌株的已知特性，提供了以下信息：（1）操纵子的精确操纵子远端边界;（2）操纵子终止子的性质;和（3）导致lacZ翻译起始intrp融合mRNA抑制的信使RNA序列。
DNA fragments containing deletions that fuse thetrpoperon to thelacoperon were cloned and the end-points of the fusions were determined. The results from DNA sequence analysis correlated well with those from genetic, biochemical and physiological studies previously reported. The sequence data from this study, in combination with the known properties of these fusion strains, provided information on: (1) the preciselacoperon distal boundary of thelacoperator; (2) the nature of thetrpoperon terminator; and (3) the messenger RNA sequences that result in inhibition oflacZtranslation initiation intrp-lacfused mRNAs.