Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling.
Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling.
复制标题
DOI：
10.1002/mabi.201500107
复制
发表时间：
2015-04
影响因子：
4.6
通讯作者：
Jaione Valle;Saioa Burgui;D. Langheinrich;C. Gil;C. Solano;Alejandro Toledo-Arana;R. Helbig;A. Lasagni;Í. Lasa
中科院分区：
文献类型：
--
作者：
Jaione Valle;Saioa Burgui;D. Langheinrich;C. Gil;C. Solano;Alejandro Toledo-Arana;R. Helbig;A. Lasagni;Í. Lasa
生物材料表面形貌的修饰是防止细菌粘附和生物膜形成的一种有前途的策略。在这项研究中，我们使用直接激光干涉图案化（DLIP）修改聚苯乙烯表面形貌在亚微米尺度。结果表明，三维微米结构对细菌粘附有深远的影响。因此，线状和柱状图案增强了S。金黄色葡萄球菌粘附，而复杂的板层微地形减少S.金黄色葡萄球菌粘附在静态和连续流动培养条件。有趣的是，片状纹理表面保留了抑制S。当表面涂覆有人血清蛋白时以及当材料皮下植入异物相关感染模型中时，金黄色葡萄球菌粘附。
Modification of the biomaterial surface topography is a promising strategy to prevent bacterial adhesion and biofilm formation. In this study, we use direct laser interference patterning (DLIP) to modify polystyrene surface topography at sub-micrometer scale. The results revealed that three-dimensional micrometer structures have a profound impact on bacterial adhesion. Thus, line- and pillar-like patterns enhanced S. aureus adhesion, whereas complex lamella microtopography reduced S. aureus adhesion in static and continuous flow culture conditions. Interestingly, lamella-like textured surfaces retained the capacity to inhibit S. aureus adhesion both when the surface is coated with human serum proteins and when the material is implanted subcutaneously in a foreign-body associated infection model.